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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA310536 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit Monoclonal antibody against Gli-1 (GLI1)
- Antibody type
- Monoclonal
- Description
- Rabbit Monoclonal antibody against Gli-1 (GLI1)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GLI1
- Isotype
- IgG
- Antibody clone number
- EPR4523
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Pharmacological targeting of GLI1 inhibits proliferation, tumor emboli formation and in vivo tumor growth of inflammatory breast cancer cells.
Oladapo HO, Tarpley M, Sauer SJ, Addo KA, Ingram SM, Strepay D, Ehe BK, Chdid L, Trinkler M, Roques JR, Darr DB, Fleming JM, Devi GR, Williams KP
Cancer letters 2017 Dec 28;411:136-149
Cancer letters 2017 Dec 28;411:136-149
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot - Anti-Gli1 antibody [EPR4523]; All lanes : Anti-Gli1 antibody [EPR4523] at 1/1000 dilution.Lane 1 : RMS13 cell lysate.Lane 2 : A673 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP-conjugated goat anti-rabbit IgG polyclonal at 1/2000 dilution.Predicted band size : 118 kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry/ Immunofluorescence - Anti-Gli1 antibody [EPR4523]; Immunofluorescent analysis of A673 cells labelling Gli1 with TA310536 at 1/250 dilution.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling Gli1 with TA310536 at 1/250 dilution.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gli1 antibody [EPR4523]; Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Gli1 with TA310536 at 1/250 dilution.
- Validation comment
- IHC
