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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [28]
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- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- NB600-600 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-600, RRID:AB_2111758
- Product name
- Rabbit Polyclonal GLI-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. No reaction occurs with human or mouse Gli2 or Gli3.
- Reactivity
- Human, Mouse, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Noncalcemic Vitamin D Hydroxyderivatives Inhibit Human Oral Squamous Cell Carcinoma and Down-regulate Hedgehog and WNT/β-Catenin Pathways.
The transcription factor GLI1 cooperates with the chromatin remodeler SMARCA2 to regulate chromatin accessibility at distal DNA regulatory elements.
Smooth Muscle Cell Reprogramming in Aortic Aneurysms.
Direct repression of anoctamin 1 (ANO1) gene transcription by Gli proteins.
Hedgehog signaling promotes tumor-associated macrophage polarization to suppress intratumoral CD8+ T cell recruitment.
Hedgehog/Gli1 signaling pathway regulates MGMT expression and chemoresistance to temozolomide in human glioblastoma.
Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis.
Nanog-driven cell-reprogramming and self-renewal maintenance in Ptch1 (+/-) granule cell precursors after radiation injury.
Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis.
Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target.
Six2creFrs2α knockout mice are a novel model of renal cystogenesis.
The sonic hedgehog signaling pathway contributes to the development of salivary gland neoplasms regardless of perineural infiltration.
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation.
Nuclear Factor of Activated T Cells-dependent Down-regulation of the Transcription Factor Glioma-associated Protein 1 (GLI1) Underlies the Growth Inhibitory Properties of Arachidonic Acid.
Gli Transcription Factors Mediate the Oncogenic Transformation of Prostate Basal Cells Induced by a Kras-Androgen Receptor Axis.
Modulation of the IL-6 Receptor α Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenström Macroglobulinemia Cells.
Screening for tumor suppressors: Loss of ephrin receptor A2 cooperates with oncogenic KRas in promoting lung adenocarcinoma.
Expression of Gli1 and Wnt2B correlates with progression and clinical outcome of pancreatic cancer.
The transcription factor GLI1 interacts with SMAD proteins to modulate transforming growth factor β-induced gene expression in a p300/CREB-binding protein-associated factor (PCAF)-dependent manner.
Hedgehog-GLI signaling inhibition suppresses tumor growth in squamous lung cancer.
Hedgehog signaling regulates telomerase reverse transcriptase in human cancer cells.
Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification.
Hedgehog signaling inhibition by the small molecule smoothened inhibitor GDC-0449 in the bone forming prostate cancer xenograft MDA PCa 118b.
Blocking Hedgehog survival signaling at the level of the GLI genes induces DNA damage and extensive cell death in human colon carcinoma cells.
Expression of hedgehog pathway components in prostate carcinoma microenvironment: shifting the balance towards autocrine signalling.
Hedgehog signaling drives cellular survival in human colon carcinoma cells.
Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment.
Indian hedgehog and beta-catenin signaling: role in the sebaceous lineage of normal and neoplastic mammalian epidermis.
Oak ASW, Bocheva G, Kim TK, Brożyna AA, Janjetovic Z, Athar M, Tuckey RC, Slominski AT
Anticancer research 2020 May;40(5):2467-2474
Anticancer research 2020 May;40(5):2467-2474
The transcription factor GLI1 cooperates with the chromatin remodeler SMARCA2 to regulate chromatin accessibility at distal DNA regulatory elements.
Safgren SL, Olson RLO, Vrabel AM, Almada LL, Marks DL, Hernandez-Alvarado N, Gaspar-Maia A, Fernandez-Zapico ME
The Journal of biological chemistry 2020 Jun 26;295(26):8725-8735
The Journal of biological chemistry 2020 Jun 26;295(26):8725-8735
Smooth Muscle Cell Reprogramming in Aortic Aneurysms.
Chen PY, Qin L, Li G, Malagon-Lopez J, Wang Z, Bergaya S, Gujja S, Caulk AW, Murtada SI, Zhang X, Zhuang ZW, Rao DA, Wang G, Tobiasova Z, Jiang B, Montgomery RR, Sun L, Sun H, Fisher EA, Gulcher JR, Fernandez-Hernando C, Humphrey JD, Tellides G, Chittenden TW, Simons M
Cell stem cell 2020 Apr 2;26(4):542-557.e11
Cell stem cell 2020 Apr 2;26(4):542-557.e11
Direct repression of anoctamin 1 (ANO1) gene transcription by Gli proteins.
Mazzone A, Gibbons SJ, Eisenman ST, Strege PR, Zheng T, D'Amato M, Ordog T, Fernandez-Zapico ME, Farrugia G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6632-6642
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6632-6642
Hedgehog signaling promotes tumor-associated macrophage polarization to suppress intratumoral CD8+ T cell recruitment.
Petty AJ, Li A, Wang X, Dai R, Heyman B, Hsu D, Huang X, Yang Y
The Journal of clinical investigation 2019 Dec 2;129(12):5151-5162
The Journal of clinical investigation 2019 Dec 2;129(12):5151-5162
Hedgehog/Gli1 signaling pathway regulates MGMT expression and chemoresistance to temozolomide in human glioblastoma.
Wang K, Chen D, Qian Z, Cui D, Gao L, Lou M
Cancer cell international 2017;17:117
Cancer cell international 2017;17:117
Human CAFs promote lymphangiogenesis in ovarian cancer via the Hh-VEGF-C signaling axis.
Wei R, Lv M, Li F, Cheng T, Zhang Z, Jiang G, Zhou Y, Gao R, Wei X, Lou J, Wu X, Luo D, Ma X, Jiang J, Ma D, Xi L
Oncotarget 2017 Sep 15;8(40):67315-67328
Oncotarget 2017 Sep 15;8(40):67315-67328
Nanog-driven cell-reprogramming and self-renewal maintenance in Ptch1 (+/-) granule cell precursors after radiation injury.
Tanno B, Leonardi S, Babini G, Giardullo P, De Stefano I, Pasquali E, Saran A, Mancuso M
Scientific reports 2017 Oct 27;7(1):14238
Scientific reports 2017 Oct 27;7(1):14238
Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis.
Xu T, Zhang H, Park SS, Venneti S, Kuick R, Ha K, Michael LE, Santi M, Uchida C, Uchida T, Srinivasan A, Olson JM, Dlugosz AA, Camelo-Piragua S, Rual JF
Neoplasia (New York, N.Y.) 2017 Mar;19(3):216-225
Neoplasia (New York, N.Y.) 2017 Mar;19(3):216-225
Gli1+ Mesenchymal Stromal Cells Are a Key Driver of Bone Marrow Fibrosis and an Important Cellular Therapeutic Target.
Schneider RK, Mullally A, Dugourd A, Peisker F, Hoogenboezem R, Van Strien PMH, Bindels EM, Heckl D, Büsche G, Fleck D, Müller-Newen G, Wongboonsin J, Ventura Ferreira M, Puelles VG, Saez-Rodriguez J, Ebert BL, Humphreys BD, Kramann R
Cell stem cell 2017 Jun 1;20(6):785-800.e8
Cell stem cell 2017 Jun 1;20(6):785-800.e8
Six2creFrs2α knockout mice are a novel model of renal cystogenesis.
Puri P, Bushnell D, Schaefer CM, Bates CM
Scientific reports 2016 Nov 17;6:36736
Scientific reports 2016 Nov 17;6:36736
The sonic hedgehog signaling pathway contributes to the development of salivary gland neoplasms regardless of perineural infiltration.
Vidal MT, Lourenço SV, Soares FA, Gurgel CA, Studart EJ, Valverde Lde F, Araújo IB, Ramos EA, Xavier FC, Dos Santos JN
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9587-601
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9587-601
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation.
Valverde Lde F, Pereira Tde A, Dias RB, Guimarães VS, Ramos EA, Santos JN, Gurgel Rocha CA
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9233-41
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9233-41
Nuclear Factor of Activated T Cells-dependent Down-regulation of the Transcription Factor Glioma-associated Protein 1 (GLI1) Underlies the Growth Inhibitory Properties of Arachidonic Acid.
Comba A, Almada LL, Tolosa EJ, Iguchi E, Marks DL, Vara Messler M, Silva R, Fernandez-Barrena MG, Enriquez-Hesles E, Vrabel AL, Botta B, Di Marcotulio L, Ellenrieder V, Eynard AR, Pasqualini ME, Fernandez-Zapico ME
The Journal of biological chemistry 2016 Jan 22;291(4):1933-47
The Journal of biological chemistry 2016 Jan 22;291(4):1933-47
Gli Transcription Factors Mediate the Oncogenic Transformation of Prostate Basal Cells Induced by a Kras-Androgen Receptor Axis.
Wu M, Ingram L, Tolosa EJ, Vera RE, Li Q, Kim S, Ma Y, Spyropoulos DD, Beharry Z, Huang J, Fernandez-Zapico ME, Cai H
The Journal of biological chemistry 2016 Dec 2;291(49):25749-25760
The Journal of biological chemistry 2016 Dec 2;291(49):25749-25760
Modulation of the IL-6 Receptor α Underlies GLI2-Mediated Regulation of Ig Secretion in Waldenström Macroglobulinemia Cells.
Jackson DA, Smith TD, Amarsaikhan N, Han W, Neil MS, Boi SK, Vrabel AM, Tolosa EJ, Almada LL, Fernandez-Zapico ME, Elsawa SF
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 15;195(6):2908-16
Journal of immunology (Baltimore, Md. : 1950) 2015 Sep 15;195(6):2908-16
Screening for tumor suppressors: Loss of ephrin receptor A2 cooperates with oncogenic KRas in promoting lung adenocarcinoma.
Yeddula N, Xia Y, Ke E, Beumer J, Verma IM
Proceedings of the National Academy of Sciences of the United States of America 2015 Nov 24;112(47):E6476-85
Proceedings of the National Academy of Sciences of the United States of America 2015 Nov 24;112(47):E6476-85
Expression of Gli1 and Wnt2B correlates with progression and clinical outcome of pancreatic cancer.
Jiang H, Li F, He C, Wang X, Li Q, Gao H
International journal of clinical and experimental pathology 2014;7(7):4531-8
International journal of clinical and experimental pathology 2014;7(7):4531-8
The transcription factor GLI1 interacts with SMAD proteins to modulate transforming growth factor β-induced gene expression in a p300/CREB-binding protein-associated factor (PCAF)-dependent manner.
Nye MD, Almada LL, Fernandez-Barrena MG, Marks DL, Elsawa SF, Vrabel A, Tolosa EJ, Ellenrieder V, Fernandez-Zapico ME
The Journal of biological chemistry 2014 May 30;289(22):15495-506
The Journal of biological chemistry 2014 May 30;289(22):15495-506
Hedgehog-GLI signaling inhibition suppresses tumor growth in squamous lung cancer.
Huang L, Walter V, Hayes DN, Onaitis M
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Mar 15;20(6):1566-75
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Mar 15;20(6):1566-75
Hedgehog signaling regulates telomerase reverse transcriptase in human cancer cells.
Mazumdar T, Sandhu R, Qadan M, DeVecchio J, Magloire V, Agyeman A, Li B, Houghton JA
PloS one 2013;8(9):e75253
PloS one 2013;8(9):e75253
Activation of Hedgehog signaling by loss of GNAS causes heterotopic ossification.
Regard JB, Malhotra D, Gvozdenovic-Jeremic J, Josey M, Chen M, Weinstein LS, Lu J, Shore EM, Kaplan FS, Yang Y
Nature medicine 2013 Nov;19(11):1505-12
Nature medicine 2013 Nov;19(11):1505-12
Hedgehog signaling inhibition by the small molecule smoothened inhibitor GDC-0449 in the bone forming prostate cancer xenograft MDA PCa 118b.
Karlou M, Lu JF, Wu G, Maity S, Tzelepi V, Navone NM, Hoang A, Logothetis CJ, Efstathiou E
The Prostate 2012 Nov;72(15):1638-47
The Prostate 2012 Nov;72(15):1638-47
Blocking Hedgehog survival signaling at the level of the GLI genes induces DNA damage and extensive cell death in human colon carcinoma cells.
Mazumdar T, Devecchio J, Agyeman A, Shi T, Houghton JA
Cancer research 2011 Sep 1;71(17):5904-14
Cancer research 2011 Sep 1;71(17):5904-14
Expression of hedgehog pathway components in prostate carcinoma microenvironment: shifting the balance towards autocrine signalling.
Tzelepi V, Karlou M, Wen S, Hoang A, Logothetis C, Troncoso P, Efstathiou E
Histopathology 2011 Jun;58(7):1037-47
Histopathology 2011 Jun;58(7):1037-47
Hedgehog signaling drives cellular survival in human colon carcinoma cells.
Mazumdar T, DeVecchio J, Shi T, Jones J, Agyeman A, Houghton JA
Cancer research 2011 Feb 1;71(3):1092-102
Cancer research 2011 Feb 1;71(3):1092-102
Hedgehog signalling in colorectal tumour cells: induction of apoptosis with cyclopamine treatment.
Qualtrough D, Buda A, Gaffield W, Williams AC, Paraskeva C
International journal of cancer 2004 Jul 20;110(6):831-7
International journal of cancer 2004 Jul 20;110(6):831-7
Indian hedgehog and beta-catenin signaling: role in the sebaceous lineage of normal and neoplastic mammalian epidermis.
Niemann C, Unden AB, Lyle S, Zouboulis ChC, Toftgård R, Watt FM
Proceedings of the National Academy of Sciences of the United States of America 2003 Sep 30;100 Suppl 1:11873-80
Proceedings of the National Academy of Sciences of the United States of America 2003 Sep 30;100 Suppl 1:11873-80
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Analysis of a FFPE mouse brain section using GLI-1 antibody at 1:600 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GLI-1 Antibody [NB600-600] - Staining in canine hair follicle. Image from a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): GLI-1 Antibody [NB600-600] - Staining for GLI-1 in untreated human B cell lines (BCWM.1, MWCL-1 and RPCI-WM1) using anti-GLI-1 antibody. Rabbit IgG Isotype Control (Cat# NBP2-36463) was used as a negative control. Image courtesy of Sherine Elsawa, Northern Illinois University.
