PAB2228

antibody from Abnova Corporation

Targeting: TRDMT1 DNMT2, RNMT1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB2228

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB2228, RRID:AB_1579926

Product name

Trdmt1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of Trdmt1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells.

Okano M, Xie S, Li E
Nucleic acids research 1998 Jun 1;26(11):2536-40

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A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast.

Yoder JA, Bestor TH
Human molecular genetics 1998 Feb;7(2):279-84

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Trdmt1 polyclonal antibody (Cat # PAB2228) in HL-60 cell lysate. Trdmtl (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the Trdmt1 polyclonal antibody (Cat # PAB2228) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)