sc-20703

antibody from Santa Cruz Biotechnology

Targeting: DNMT3A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-20703

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-20703, RRID:AB_2093990

Product name

Anti-DNMT3A

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

Mature microRNAs identified in highly purified nuclei from HCT116 colon cancer cells.

Park CW, Zeng Y, Zhang X, Subramanian S, Steer CJ
RNA biology 2010 Sep-Oct;7(5):606-14

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Dynamic instability of genomic methylation patterns in pluripotent stem cells.

Ooi SK, Wolf D, Hartung O, Agarwal S, Daley GQ, Goff SP, Bestor TH
Epigenetics & chromatin 2010 Sep 24;3(1):17

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Site-specific DNA methylation by a complex of PU.1 and Dnmt3a/b

M Suzuki, T Yamada, F Kihara-Negishi, T Sakurai, E Hara, D G Tenen, N Hozumi, T Oikawa
Oncogene 2005 Dec;25(17):2477-2488

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Band of predicted size in kDa (+/-20%) with additional bands present.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 102

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.