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Western blot
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- Antibody Data
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- References [4]
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [1]
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- Product number
- GTX129125 - Provider product page
- Provider
- GeneTex
- Product name
- DNMT3A antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Epigenetic targeting DNMT1 of pancreatic ductal adenocarcinoma using interstitial control release biodegrading polymer reduced tumor growth through hedgehog pathway inhibition.
The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Rhein reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal fibrosis in mice.
Elevation of soluble guanylate cyclase suppresses proliferation and survival of human breast cancer cells.
Huang MH, Chou YW, Li MH, Shih TE, Lin SZ, Chuang HM, Chiou TW, Su HL, Harn HJ
Pharmacological research 2019 Jan;139:50-61
Pharmacological research 2019 Jan;139:50-61
The inhibitory activity of gallic acid against DNA methylation: application of gallic acid on epigenetic therapy of human cancers.
Weng YP, Hung PF, Ku WY, Chang CY, Wu BH, Wu MH, Yao JY, Yang JR, Lee CH
Oncotarget 2018 Jan 2;9(1):361-374
Oncotarget 2018 Jan 2;9(1):361-374
Rhein reversal of DNA hypermethylation-associated Klotho suppression ameliorates renal fibrosis in mice.
Zhang Q, Yin S, Liu L, Liu Z, Cao W
Scientific reports 2016 Oct 5;6:34597
Scientific reports 2016 Oct 5;6:34597
Elevation of soluble guanylate cyclase suppresses proliferation and survival of human breast cancer cells.
Wen HC, Chuu CP, Chen CY, Shiah SG, Kung HJ, King KL, Su LC, Chang SC, Chang CH
PloS one 2015;10(4):e0125518
PloS one 2015;10(4):e0125518
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3A antibody detects DNMT3A protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and blotted with DNMT3A antibody (GTX129125) diluted by 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129125) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3A antibody detects DNMT3A protein at nucleus and cytoplasm by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNMT3A protein stained by DNMT3A antibody (GTX129125) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3A antibody immunoprecipitates DNMT3A protein in IP experiments.IP samples: 293T nuclear extractA. 50 £gg 293T nuclear extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of DNMT3A protein by 4 £gg DNMT3A antibody (GTX129125)5 % SDS-PAGEThe immunoprecipitated DNMT3A protein was detected by DNMT3A antibody (GTX129125) diluted at 1:2000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of DNMT3A protein from 293T whole cell extracts using 5 £gg of DNMT3A antibody (GTX129125) or DNMT3A antibody (GTX129126).Western blot analysis was performed using DNMT3A antibody (GTX129125).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3A antibody detects DNMT3A protein at nucleus on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. DNMT3A antibody (GTX129125) dilution: 1:500.
