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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB0080 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0080, RRID:AB_1017129
- Product name
- Dnmt3a monoclonal antibody, clone 64B1446
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against full length recombinant Dnmt3a.
- Isotype
- IgG
- Antibody clone number
- 64B1446
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Arsenic induces functional re-expression of estrogen receptor α by demethylation of DNA in estrogen receptor-negative human breast cancer.
Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres.
Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation.
Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development.
Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing.
Coordinate regulation of DNA methyltransferase expression during oogenesis.
Du J, Zhou N, Liu H, Jiang F, Wang Y, Hu C, Qi H, Zhong C, Wang X, Li Z
PloS one 2012;7(4):e35957
PloS one 2012;7(4):e35957
Multiplex rt-PCR expression analysis of developmentally important genes in individual mouse preimplantation embryos and blastomeres.
May A, Kirchner R, Müller H, Hartmann P, El Hajj N, Tresch A, Zechner U, Mann W, Haaf T
Biology of reproduction 2009 Jan;80(1):194-202
Biology of reproduction 2009 Jan;80(1):194-202
Helicobacter pylori regulates p21(WAF1) by histone H4 acetylation.
Xia G, Schneider-Stock R, Diestel A, Habold C, Krueger S, Roessner A, Naumann M, Lendeckel U
Biochemical and biophysical research communications 2008 May 2;369(2):526-31
Biochemical and biophysical research communications 2008 May 2;369(2):526-31
Maternal and zygotic Dnmt1 are necessary and sufficient for the maintenance of DNA methylation imprints during preimplantation development.
Hirasawa R, Chiba H, Kaneda M, Tajima S, Li E, Jaenisch R, Sasaki H
Genes & development 2008 Jun 15;22(12):1607-16
Genes & development 2008 Jun 15;22(12):1607-16
Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing.
Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R
Genes & development 2007 Dec 1;21(23):3110-22
Genes & development 2007 Dec 1;21(23):3110-22
Coordinate regulation of DNA methyltransferase expression during oogenesis.
Lucifero D, La Salle S, Bourc'his D, Martel J, Bestor TH, Trasler JM
BMC developmental biology 2007 Apr 19;7:36
BMC developmental biology 2007 Apr 19;7:36
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Dnmt3a in (A) Dnmt3a transfected 293 cell lysate and (B) untransfected 293 cell lysate. Using Dnmt3a monoclonal antibody, clone 64B1446 (Cat # MAB0080) at 1 ug/mL .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immuno-staining of Dnmt3a. Using Dnmt3a monoclonal antibody, clone 64B1446 (Cat # MAB0080) at 5 ug/mL dilution on cultured HeLa cells. The nucleic staining is shown in most of the cells.
- Validation comment
- Immunocytochemistry
