Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-56521 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56521, RRID:AB_838135
- Product name
- Mouse Monoclonal DNMT3A Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The orphan nuclear receptor GCNF recruits DNA methyltransferase for Oct-3/4 silencing.
Sato N, Kondo M, Arai K
Biochemical and biophysical research communications 2006 Jun 9;344(3):845-51
Biochemical and biophysical research communications 2006 Jun 9;344(3):845-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Dnmt3a in (A) Dnmt3a transfected and (B) untransfected 293 cell lysate using Dnmt3a antibody at 1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Western Blot for Dnmt3a in mouse brain cells. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis using the Biotin conjugate of NB100-56521. Detection of Dnmt3a using NB100-55782 at 2 ug/ml against 10 ug of 293 cell lysate transfected with either mouse Dnmt3a (Lanes 1) or mouse Dnmt3b (Lanes 2).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): DNMT3A Antibody (64B814.1) [NB100-56521] - Analysis of Alexa Fluor (R) 488 conjugate of NB100-56521. An intracellular stain was performed on HeLa cells with DNMT3A antibody (64B814.1) NB100-56521AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange).