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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- BS-3879R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytochrome b245 Light Chain Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- This image was generously provided by Ao Li, MD from Chongqing University of Technology. Serum-starved HK-2 cells were pretreated with DMSO (vehicle) or ATG (2 µM) for 4 h, and then incubated with 10 ng/ml TGF-β1 for 0.25-24 hr.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Paraformaldehyde-fixed, paraffin embedded human liver cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Cytochrome b245 Light Chain Polyclonal Antibody, Unconjugated (bs-3879R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
