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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
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- Product number
- GTX628894 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX628894, RRID:AB_2773724
- Product name
- Kaiso antibody [EPR4668]
- Antibody type
- Monoclonal
- Host
- Rabbit
- Vial size
- 100 µl
Submitted references Stress-induced release of Oct-1 from the nuclear envelope is mediated by JNK phosphorylation of lamin B1.
Boubriak II, Malhas AN, Drozdz MM, Pytowski L, Vaux DJ
PloS one 2017;12(5):e0177990
PloS one 2017;12(5):e0177990
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GFP antibody detects GFP protein by Western blot analysis.A. 30 µg 293T whole cell extractB. 30 µg 293T whole cell extract expressing GFP-tagged protein C. 30 µg 293T whole cell extract expressing RFP-tagged protein 12 % SDS-PAGEGFP antibody (GTX628894) dilution: 1:10000
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ELISA detection of GFP using GTX628894 for capture and GTX113617 for detection.Coating Ab: mouse anti-GFP (GTX628894, 2μg/well )Sample: GFP proteinDetected Ab: rabbit anti-GFP (GTX113617)Conjμgated Ab: goat anti-rabbit IgG-HRP (GTX213110-01, 1:20000)
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GFP tag antibody immunoprecipitates GFP-fused protein in IP experiments. IP Sample: 400 £gg cell lysate/extract of GFP-fused gene transfected 293T cells A. 30 ug lysate/extract of transfected 293T cell B. Control with 2 £gg of preimmune mouse IgG C. Immunoprecipitation of GFP-fused protein by 2 £gg of GFP tag antibody (GTX628894) 12% SDS-PAGE The immunoprecipitated GFP-fused protein was detected by GFP tag antibody (GTX628894) diluted at 1:1000. EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.
