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LearnH00010009-M01
antibody from Novus Biologicals
Targeting: ZBTB33
kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, ZNF348
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00010009-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010009-M01, RRID:AB_539978
- Product name
- Mouse Monoclonal Kaiso Antibody
- Antibody type
- Monoclonal
- Description
- Ascites. ZBTB33 - zinc finger and BTB domain containing 33 (2B2)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.05 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kaiso Antibody (2B2) [H00010009-M01] - Analysis of ZBTB33 over-expressed 293 cell line, cotransfected with ZBTB33 Validated Chimera RNAi ( Cat # H00010009-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZBTB33 monoclonal antibody (M01), clone 2B2 (Cat # H00010009-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Kaiso Antibody (2B2) [H00010009-M01] - Analysis of ZBTB33 expression in transfected 293T cell line by ZBTB33 monoclonal antibody (M01), clone 2B2.Lane 1: ZBTB33 transfected lysate(74.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Kaiso Antibody (2B2) [H00010009-M01] - Detection limit for recombinant GST tagged ZBTB33 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Kaiso Antibody (2B2) [H00010009-M01] - Analysis of monoclonal antibody to ZBTB33 on HeLa cell . Antibody concentration 10 ug/ml.
