NB100-1903

antibody from Novus Biologicals

Targeting: FURIN FUR, PACE, PCSK3, SPC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

NB100-1903

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1903, RRID:AB_2247069

Product name

Rabbit Polyclonal Furin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects Furin convertase from canine and mouse cells as well as transfected human Furin. This does not detect endogenous Furin from BSC-40, HeLa, J774A.1 BPAEC, or CHO cells nor from rat skeletal muscle, spleen, kidney, ovary, testes, heart, or brain tissues.

Reactivity

Human, Mouse, Rat, Canine, Hamster, Porcine, Simian

Host

Rabbit

Antigen sequence

Amino acids 781-794.


Isotype

IgG

Vial size

100 uL

Concentration

2 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Furin Antibody [NB100-1903] - Analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), HEK 293 (Lane 2), K-562 (Lane 3), SH-SY5Y (Lane 4) and 10uL conditioned media from HeLa cell line (Lane 5).

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: Furin Antibody [NB100-1903] - Flow cytometry analysis of Furin Convertase was done on HeLa cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Furin Convertase Rabbit Polyclonal Antibody or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor (R) 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10, 000 cells were acquired and analyzed for each sample using an Attune (R) Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.