GTX22862

antibody from GeneTex

Targeting: CACNA1S CACNL1A3, Cav1.1, HOKPP, hypoPP, MHS5

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX22862

Provider

GeneTex

Proper citation

GeneTex Cat#GTX22862, RRID:AB_384882

Product name

Cav1.1 antibody [1A]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Guinea Pig, Rabbit

Host

Mouse

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry was performed on normal biopsies of deparaffinized human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with or without Cav1.1 antibody [1A] overnight at 4¢XC in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of Dihydropyridine Receptor alpha-2 showing positive staining in the membrane and cytoplasm of C6 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Dihydropyridine Receptor alpha-2 monoclonal antibody (GTX22864) at a dilution of 1:100 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Validation comment

FACS

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometry analysis of Dihydropyridine Receptor alpha-2 showing positive staining in the membrane and cytoplasm of SH-SY5Y cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Dihydropyridine Receptor alpha-2 monoclonal antibody (GTX22864) at a dilution of 1:100 for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Validation comment

FACS