PA5-110384

antibody from Invitrogen Antibodies

Targeting: SLC25A27 FLJ33552, UCP4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-110384

Provider

Invitrogen Antibodies

Product name

UCP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.75 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

In vivo magnetic resonance spectroscopy in the brain of Cdkl5 null mice reveals a metabolic profile indicative of mitochondrial dysfunctions.

Carli S, Chaabane L, Butti C, De Palma C, Aimar P, Salio C, Vignoli A, Giustetto M, Landsberger N, Frasca A
Journal of neurochemistry 2021 May;157(4):1253-1269

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UCP4 in mouse brain. Samples were incubated in polyclonal UCP4 antibody (Product # PA5-110384) using a dilution of 1:3000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of UCP4 in NIH/3T3 cells. Samples were incubated in polyclonal UCP4 antibody (Product # PA5-110384) using a dilution of 1:100, followed by DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

6 FIGURE Cdkl5 KO brains display an aberrant expression of complex IV of the electron transport chain. (a-c) The transcriptional expression of mitochondrial genes was analyzed by quantitative RT-PCR in WT and KO hippocampi ( n = 15 WT and 8 KO). Results displayed a consistent reduction of Cox7b and Slc25a27 , while Slc25a12 showed a decreasing trend. Hprt was used as internal standard. The fold changes of transcript levels, compared to WT animals, are reported as mean +- SEM ( $ p < .068; * p < .05). (d-f) Western blots of mitochondrial proteins from WT and KO mice. A significant decrease was confirmed for the complex IV of the ETC, Cox7b. Bar graphs show the mean +- SEM of the expression levels in Cdkl5 KO compared to WT, of ETCs (total OXPHOS) ( n = 10 WT, n = 9 KO), Slc25a27 (UCP4) ( n = 9 WT, n = 9 KO), and Slc25a12 (Aralar) ( n = 6 WT, n = 6 KO). Full blots are reported in Figure S1. Statistical significance was assessed by Student's t test ( Ndufv2, Sdha, Uqrc1, Atp5a1 , Slc25a27, and Slc25a12 in quantitative RT-PCR and ETC complexes and Slc25a12 in Western Blot ) or Mann-Whitney U test ( Cox7b in quantitative RT-PCR and Slc25a27 in Western Blot), in accordance with D'Agostino-Pearson test for data distribution; * p < .05