Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX129291 - Provider product page
- Provider
- GeneTex
- Product name
- RFC1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RFC1 antibody detects RFC1 protein by western blot analysis.A. 30 £gg 293T whole cell lysate/extract B. 30 £gg A431 whole cell lysate/extract C. 30 £gg HeLa whole cell lysate/extract D. 30 £gg HepG2 whole cell lysate/extract5 % SDS-PAGERFC1 antibody (GTX129291) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RFC1 antibody detects RFC1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: RFC1 protein stained by RFC1 antibody (GTX129291) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RFC1 antibody immunoprecipitates RFC1 protein in IP experiments.IP samples: HeLa whole cell extractA. 35 £gg HeLa whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of RFC1 protein by 4 £gg RFC1 antibody (GTX129291)D. Immunoprecipitation of RFC1 protein by 4 £gg RFC1 antibody (GTX129292) 5 % SDS-PAGEThe immunoprecipitated RFC1 protein was detected by RFC1 antibody (GTX129291) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
