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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- CF502677 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V1B1 Monoclonal Antibody (OTI1H6), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1H6
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (RC209462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP. Positive lysates LY400635 (100 µg) and LC400635 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B1 (RC209462, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP. Positive lysates LY400635 (100 µg) and LC400635 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-ATP6V1B1 mouse monoclonal antibody (TA502677) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ATP6V1B1(RC209462).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ATP6V1B1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502677)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-ATP6V1B1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502677)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC209462 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ATP6V1B1 antibody (TA502677), and then analyzed by flow cytometry.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells, using anti-ATP6V1B1 antibody (TA502677), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
