ABIN950569

antibody from antibodies-online

Targeting: ATP6V1B1 ATP6B1, RTA1B, VATB, Vma2, VPP3

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN950569

Provider

antibodies-online

Product name

anti-ATPase, H+ Transporting, Lysosomal 56/58kDa, V1 Subunit B1 (ATP6V1B1) (AA 291-318), (Middle Region) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 291-318 amino acids from the Central region of human ATP6V1B1

Description

Affinity chromatography on Protein A

Reactivity

Human

Host

Rabbit

Epitope

AA 291-318,Middle Region

Vial size

0.4 mL

Concentration

0.25 mg/mL

Storage

Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S, DREAM investigators
Diabetes care 2010 Oct;33(10):2250-3

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Distal renal tubular acidosis and its relationship with hearing loss in children: preliminary report.

Sharifian M, Esfandiar N, Mazaheri S, Kariminejad A, Mohkam M, Dalirani R, Esmaili R, Ahmadi M, Hassas-Yeganeh M
Iranian journal of kidney diseases 2010 Jul;4(3):202-6

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Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD, ASCOT investigators, NORDIL investigators, BRIGHT Consortium
American journal of human genetics 2009 Nov;85(5):628-42

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Inner ear abnormalities in four patients with dRTA and SNHL: clinical and genetic heterogeneity.

Andreucci E, Bianchi B, Carboni I, Lavoratti G, Mortilla M, Fonda C, Bigozzi M, Genuardi M, Giglio S, Pela I
Pediatric nephrology (Berlin, Germany) 2009 Nov;24(11):2147-53

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Genetic studies in a family with distal renal tubular acidosis and sensorineural deafness.

Sethi SK, Singh N, Gil H, Bagga A
Indian pediatrics 2009 May;46(5):425-7

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

ATP6V1B1 Antibody (Center) (Cat. #AP50306PU-N) western blot analysis in WiDr,K562 cell line lysates (35μg/lane).This demonstrates the ATP6V1B1 antibody detected the ATP6V1B1 protein (arrow).

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of ATP6V1B1 Antibody (Center)(Cat#AP50306PU-N) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

ATP6V1B1 Antibody (Center) (Cat. #AP50306PU-N)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ATP6V1B1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.