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- Product number
- A00404-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-T-bet/Tbx21 Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for T-bet/Tbx21 detection. Tested with WB, FCM in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Impaired TNF/TNFR2 signaling enhances Th2 and Th17 polarization and aggravates allergic airway inflammation.
Li XM, Chen X, Gu W, Guo YJ, Cheng Y, Peng J, Guo XJ
American journal of physiology. Lung cellular and molecular physiology 2017 Sep 1;313(3):L592-L601
American journal of physiology. Lung cellular and molecular physiology 2017 Sep 1;313(3):L592-L601
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of TBX21 using anti-TBX21 antibody (A00404-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TBX21 antigen affinity purified polyclonal antibody (Catalog # A00404-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TBX21 at approximately 65KD. The expected band size for TBX21 is at 58KD.
- Additional image
