Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- V4514 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- T-bet Antibody / Tbox-bet / TBX21
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- TBX21/6724
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- Aliquot the TBX21 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
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- NSJ Bioreagents (provider)
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- Experimental details
- IHC staining of FFPE human colon carcinoma tissue with TBX21 antibody (clone TBX21/6724). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.