PA5-117801

antibody from Invitrogen Antibodies

Targeting: ATP6V1C1 ATP6C, ATP6D, VATC, Vma5

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117801

Provider

Invitrogen Antibodies

Product name

V-ATPase C1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of V-ATPase C1 in Lane A: HepG2 Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117801) with a dilution of 1:10,000 , followed by Goat Anti-Rabbit IgG (H+L)/HRP using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 44 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using V-ATPase C1 Polyclonal Antibody (Product # PA5-117801) at 1:10,000 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

V-ATPase C1 Immunoprecipitation using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate 4 µL with V-ATPase C1 Polyclonal Antibody (Product # PA5-117801) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: V-ATPase C1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 47 kDa. Observed band size: 50 kDa.