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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [6]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX114056 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114056, RRID:AB_10721051
- Product name
- ATP6V1E1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A549 12% SDS PAGE GTX114056 diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse brain 12% SDS PAGE GTX114056 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ATP6V1E1 antibody (GTX114056) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ATP6V1E1 antibody (GTX114056) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ATP6V1E1 antibody (GTX114056) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with ATP6V1E1 antibody (GTX114056) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATP6V1E1 antibody detects ATP6V1E1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: ATP6V1E1 protein stained by ATP6V1E1 antibody (GTX114056) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ATP6V1E1(GTX114056) antibody at 1:250 dilution.
