LS-B11773

antibody from LSBio

Targeting: ATP6V1F ATP6S14, VATF, Vma7

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B11773

Provider

LSBio

Product name

IHC-plus™ ATP6V1F Antibody (clone 5F10) LS-B11773

Antibody type

Monoclonal

Description

Protein A/G purified

Reactivity

Human, Rat, Canine, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

5F10

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1F.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of extracts (35 ug) from 9 different cell lines by using anti-ATP6V1F monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).