NBP2-03498

antibody from Novus Biologicals

Targeting: ATP6V1F ATP6S14, VATF, Vma7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-03498

Provider

Novus Biologicals

Product name

Mouse Monoclonal V-type proton ATPase subunit F Antibody

Antibody type

Monoclonal

Description

Affinity purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY V-type proton ATPase subunit F (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-V-type proton ATPase subunit F.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human Kidney tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human liver tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lung tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human lymph node tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human prostate tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - Staining of paraffin-embedded Human thyroid tissue using anti-V-type proton ATPase subunit F mouse monoclonal antibody.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: V-type proton ATPase subunit F Antibody (1B8) [NBP2-03498] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-V-type proton ATPase subunit F antibody, and then analyzed by flow cytometry.