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LearnPA5-22134
antibody from Invitrogen Antibodies
Targeting: ATP6V1H
CGI-11, SFD, SFDalpha, SFDbeta, VMA13
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-22134 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- V-ATPase H Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, Raji, mouse brain.
- Concentration
- 0.76 mg/mL
Submitted references Combined LRRK2 mutation, aging and chronic low dose oral rotenone as a model of Parkinson's disease.
Liu HF, Ho PW, Leung GC, Lam CS, Pang SY, Li L, Kung MH, Ramsden DB, Ho SL
Scientific reports 2017 Jan 18;7:40887
Scientific reports 2017 Jan 18;7:40887
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of V-ATPase H using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a V-ATPase H polyclonal antibody (Product # PA5-22134) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of V-ATPase H using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a V-ATPase H polyclonal antibody (Product # PA5-22134) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using V-ATPase H Polyclonal Antibody (Product # PA5-22134). Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with V-ATPase H Polyclonal Antibody (Product # PA5-22134) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using V-ATPase H Polyclonal Antibody (Product # PA5-22134). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with V-ATPase H Polyclonal Antibody (Product # PA5-22134) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- V-ATPase H Polyclonal Antibody detects ATP6V1H protein at cytoplasm by immunofluorescent analysis. Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATP6V1H stained by V-ATPase H Polyclonal Antibody (Product # PA5-22134) diluted at 1:500. Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- V-ATPase H Polyclonal Antibody detects ATP6V1H protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ATP6V1H stained by V-ATPase H Polyclonal Antibody (Product # PA5-22134) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- V-ATPase H Polyclonal Antibody detects ATP6V1H protein at cytoplasm on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. V-ATPase H Polyclonal Antibody (Product # PA5-22134) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
