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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- MA5-12840 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPSA Monoclonal Antibody (MLuC5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-12840 targets Laminin Receptor in FACS, WB, ICC/IF, and IHC (P) applications and shows reactivity with Human samples.
- Antibody clone number
- MLuC5
- Concentration
- 0.2 mg/mL
Submitted references Inhibition of EMMPRIN and MMP-9 Expression by Epigallocatechin-3-Gallate through 67-kDa Laminin Receptor in PMA-Induced Macrophages.
The effects of cytotoxic necrotizing factor 1 expression in the uptake of Escherichia coli K1 by macrophages and the onset of meningitis in newborn mice.
Green tea polyphenol epigallocatechin-3-gallate inhibits TNF-α-induced production of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells.
Green tea polyphenol EGCG sensing motif on the 67-kDa laminin receptor.
Enteric-delivered rapamycin enhances resistance of aged mice to pneumococcal pneumonia through reduced cellular senescence.
Green tea polyphenol epigallocatechin-3-gallate inhibits TLR2 signaling induced by peptidoglycan through the polyphenol sensing molecule 67-kDa laminin receptor.
High expression of 67-kDa laminin receptor relates to the proliferation of leukemia cells and increases expression of GM-CSF receptor.
Epigallocatechin-3-gallate induces cell death in acute myeloid leukaemia cells and supports all-trans retinoic acid-induced neutrophil differentiation via death-associated protein kinase 2.
Relationship of up-regulation of 67-kd laminin receptor to grade of cervical intraepithelial neoplasia and to high-risk HPV types and prognosis in cervical cancer.
Relationship of up-regulation of 67-kd laminin receptor to grade of cervical intraepithelial neoplasia and to high-risk HPV types and prognosis in cervical cancer.
Increase in laminin expression in allergic airway remodelling and decrease by dexamethasone.
Human T cells express a functional ionotropic glutamate receptor GluR3, and glutamate by itself triggers integrin-mediated adhesion to laminin and fibronectin and chemotactic migration.
Wang QM, Wang H, Li YF, Xie ZY, Ma Y, Yan JJ, Gao YF, Wang ZM, Wang LS
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;39(6):2308-2319
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;39(6):2308-2319
The effects of cytotoxic necrotizing factor 1 expression in the uptake of Escherichia coli K1 by macrophages and the onset of meningitis in newborn mice.
Chang AC, Krishnan S, Prasadarao NV
Virulence 2016 Oct 2;7(7):806-18
Virulence 2016 Oct 2;7(7):806-18
Green tea polyphenol epigallocatechin-3-gallate inhibits TNF-α-induced production of monocyte chemoattractant protein-1 in human umbilical vein endothelial cells.
Wang ZM, Gao W, Wang H, Zhao D, Nie ZL, Shi JQ, Zhao S, Lu X, Wang LS, Yang ZJ
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2014;33(5):1349-58
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2014;33(5):1349-58
Green tea polyphenol EGCG sensing motif on the 67-kDa laminin receptor.
Fujimura Y, Sumida M, Sugihara K, Tsukamoto S, Yamada K, Tachibana H
PloS one 2012;7(5):e37942
PloS one 2012;7(5):e37942
Enteric-delivered rapamycin enhances resistance of aged mice to pneumococcal pneumonia through reduced cellular senescence.
Hinojosa CA, Mgbemena V, Van Roekel S, Austad SN, Miller RA, Bose S, Orihuela CJ
Experimental gerontology 2012 Dec;47(12):958-65
Experimental gerontology 2012 Dec;47(12):958-65
Green tea polyphenol epigallocatechin-3-gallate inhibits TLR2 signaling induced by peptidoglycan through the polyphenol sensing molecule 67-kDa laminin receptor.
Byun EH, Omura T, Yamada K, Tachibana H
FEBS letters 2011 Mar 9;585(5):814-20
FEBS letters 2011 Mar 9;585(5):814-20
High expression of 67-kDa laminin receptor relates to the proliferation of leukemia cells and increases expression of GM-CSF receptor.
Ando K, Miyazaki Y, Sawayama Y, Tominaga S, Matsuo E, Yamasaki R, Inoue Y, Iwanaga M, Imanishi D, Tsushima H, Fukushima T, Imaizumi Y, Taguchi J, Yoshida S, Hata T, Tomonaga M
Experimental hematology 2011 Feb;39(2):179-186.e4
Experimental hematology 2011 Feb;39(2):179-186.e4
Epigallocatechin-3-gallate induces cell death in acute myeloid leukaemia cells and supports all-trans retinoic acid-induced neutrophil differentiation via death-associated protein kinase 2.
Britschgi A, Simon HU, Tobler A, Fey MF, Tschan MP
British journal of haematology 2010 Apr;149(1):55-64
British journal of haematology 2010 Apr;149(1):55-64
Relationship of up-regulation of 67-kd laminin receptor to grade of cervical intraepithelial neoplasia and to high-risk HPV types and prognosis in cervical cancer.
Branca M, Giorgi C, Ciotti M, Santini D, Di Bonito L, Costa S, Benedetto A, Bonifacio D, Di Bonito P, Paba P, Accardi L, Mariani L, Syrjänen S, Favalli C, Syrjänen K, HPV-PathogenISS Study Group.
Acta cytologica 2006 Jan-Feb;50(1):6-15
Acta cytologica 2006 Jan-Feb;50(1):6-15
Relationship of up-regulation of 67-kd laminin receptor to grade of cervical intraepithelial neoplasia and to high-risk HPV types and prognosis in cervical cancer.
Branca M, Giorgi C, Ciotti M, Santini D, Di Bonito L, Costa S, Benedetto A, Bonifacio D, Di Bonito P, Paba P, Accardi L, Mariani L, Syrjänen S, Favalli C, Syrjänen K, HPV-PathogenISS Study Group
Acta cytologica 2006 Jan-Feb;50(1):6-15
Acta cytologica 2006 Jan-Feb;50(1):6-15
Increase in laminin expression in allergic airway remodelling and decrease by dexamethasone.
Christie PE, Jonas M, Tsai CH, Chi EY, Henderson WR Jr
The European respiratory journal 2004 Jul;24(1):107-15
The European respiratory journal 2004 Jul;24(1):107-15
Human T cells express a functional ionotropic glutamate receptor GluR3, and glutamate by itself triggers integrin-mediated adhesion to laminin and fibronectin and chemotactic migration.
Ganor Y, Besser M, Ben-Zakay N, Unger T, Levite M
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 15;170(8):4362-72
Journal of immunology (Baltimore, Md. : 1950) 2003 Apr 15;170(8):4362-72
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Laminin Receptor was performed by loading 25 µg of HepG2 (lane 1) and Hela (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Laminin Receptor monoclonal antibody (Product # MA5-12840) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~43 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Laminin Receptor (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Laminin Receptor monoclonal antibody (Product # MA5-12840) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human breast carcinoma stained with Laminin Receptor antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane with some cytoplasmic staining of tumor cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Laminin Receptor in MCF-7 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Laminin Receptor monoclonal antibody (Product # MA5-12840) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
