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- Product number
- PB9075 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-BDNF Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit Polyclonal BDNF Antibody. Validated in Flow Cytometry, IHC, ICC, WB and tested in Human, Mouse, Rat. Size: 100μg/vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Menthol-Modified Quercetin Liposomes with Brain-Targeting Function for the Treatment of Senescent Alzheimer's Disease.
Biological effects of exposure to 2650 MHz electromagnetic radiation on the behavior, learning, and memory of mice.
Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota.
The beneficial effects of vortioxetine on BDNF, CREB, S100B, β amyloid, and glutamate NR2b receptors in chronic unpredictable mild stress model of depression.
Plasminogen decreases Aβ42 and Tau deposition, and shows multi-beneficial effects on Alzheimer's disease in mice and humans.
SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.
SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells.
Study on the mechanism of visual aging in cats' primary visual cortex based on BDNF-TrkB signal pathway.
Central and peripheral effects of environmental enrichment in a mouse model of arthritis.
Agomelatine Softens Depressive-Like Behavior through the Regulation of Autophagy and Apoptosis.
Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis.
Effect of C-phycocyanin on HDAC3 and miRNA-335 in Alzheimer's disease.
Intravenously Delivered Allogeneic Mesenchymal Stem Cells Bidirectionally Regulate Inflammation and Induce Neurotrophic Effects in Distal Middle Cerebral Artery Occlusion Rats Within the First 7 Days After Stroke.
Resveratrol prevents cognitive deficits induced by chronic unpredictable mild stress: Sirt1/miR-134 signalling pathway regulates CREB/BDNF expression in hippocampus in vivo and in vitro.
Influence of hippocampal low-frequency stimulation on GABA(A) R α1, ICER and BNDF expression level in brain tissues of amygdala-kindled drug-resistant temporal lobe epileptic rats.
Abnormal expressions of AGEs, TGF-β1, BDNF and their receptors in diabetic rat colon-Associations with colonic morphometric and biomechanical remodeling.
Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord.
The Proliferation Enhancing Effects of Salidroside on Schwann Cells In Vitro.
Effect of hippocampal L‑NBP on BDNF and TrkB expression and neurological function of vascular dementia rats.
Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy.
Synergistic effects of G-CSF and bone marrow stromal cells on nerve regeneration with acellular nerve xenografts.
Electroacupuncture Ameliorates Learning and Memory and Improves Synaptic Plasticity via Activation of the PKA/CREB Signaling Pathway in Cerebral Hypoperfusion.
Ginkgo biloba and vitamin E ameliorate haloperidol-induced vacuous chewingmovement and brain-derived neurotrophic factor expression in a rat tardive dyskinesia model.
Dexmedetomidine protects against learning and memory impairments caused by electroconvulsive shock in depressed rats: Involvement of the NMDA receptor subunit 2B (NR2B)-ERK signaling pathway.
BDNF-induced expansion of cumulus-oocyte complexes in pigs was mediated by microRNA-205.
Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene.
Protocatechuic acid improves cognitive deficits and attenuates amyloid deposits, inflammatory response in aged AβPP/PS1 double transgenic mice.
Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles.
Sciatic nerve repair by acellular nerve xenografts implanted with BMSCs in rats xenograft combined with BMSCs.
Neural stem cells over-expressing brain-derived neurotrophic factor (BDNF) stimulate synaptic protein expression and promote functional recovery following transplantation in rat model of traumatic brain injury.
The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm.
Cellular and molecular mechanisms underlying the action of ginsenoside Rg1 against Alzheimer's disease.
Icariin isolated from Epimedium brevicornum Maxim attenuates learning and memory deficits induced by d-galactose in rats.
Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.
Protective effect of total flavones of Abelmoschus manihot L. Medic against poststroke depression injury in mice and its action mechanism.
Enhanced neural differentiation of neural stem cells and neurite growth by amniotic epithelial cell co-culture.
Application of bFGF and BDNF to improve angiogenesis and cardiac function.
Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats.
Moclobemide up-regulates proliferation of hippocampal progenitor cells in chronically stressed mice.
Liu WY, Yu Y, Zang J, Liu Y, Li FR, Zhang L, Guo RB, Kong L, Ma LY, Li XT
ACS chemical neuroscience 2024 Jun 5;15(11):2283-2295
ACS chemical neuroscience 2024 Jun 5;15(11):2283-2295
Biological effects of exposure to 2650 MHz electromagnetic radiation on the behavior, learning, and memory of mice.
Zheng R, Zhang X, Gao Y, Gao D, Gong W, Zhang C, Dong G, Li Z
Brain and behavior 2023 Jun;13(6):e3004
Brain and behavior 2023 Jun;13(6):e3004
Soluble dietary fiber and cellulose from Saccharina japonica by-product ameliorate Loperamide-induced constipation via modulating enteric neurotransmitters, short-chain fatty acids and gut microbiota.
Cao J, Wang K, Li N, Zhang L, Qin L, He Y, Wang J, Qu C, Miao J
International journal of biological macromolecules 2023 Jan 31;226:1319-1331
International journal of biological macromolecules 2023 Jan 31;226:1319-1331
The beneficial effects of vortioxetine on BDNF, CREB, S100B, β amyloid, and glutamate NR2b receptors in chronic unpredictable mild stress model of depression.
Ünal GÖ, Erkılınç G, Öztürk KH, Doguç DK, Özmen Ö
Psychopharmacology 2023 Dec;240(12):2499-2513
Psychopharmacology 2023 Dec;240(12):2499-2513
Plasminogen decreases Aβ42 and Tau deposition, and shows multi-beneficial effects on Alzheimer's disease in mice and humans.
Guo C, Wang T, Zhang D, Ge X, Li J
Biochemical and biophysical research communications 2023 Apr 30;654:102-111
Biochemical and biophysical research communications 2023 Apr 30;654:102-111
SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.
Wang R, Yang M, Wu Y, Liu R, Liu M, Li Q, Su X, Xin Y, Huo W, Deng Q, Ba Y, Huang H
International journal of biological macromolecules 2022 Sep 30;217:219-228
International journal of biological macromolecules 2022 Sep 30;217:219-228
SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells.
Liu T, Wang Y, Lu L, Liu Y
Journal of nanobiotechnology 2022 Mar 27;20(1):159
Journal of nanobiotechnology 2022 Mar 27;20(1):159
Study on the mechanism of visual aging in cats' primary visual cortex based on BDNF-TrkB signal pathway.
Tong C, Cao S
Scientific reports 2022 Jun 22;12(1):10576
Scientific reports 2022 Jun 22;12(1):10576
Central and peripheral effects of environmental enrichment in a mouse model of arthritis.
Estrázulas M, Freitas RDS, Käfer ET, Dagnino APA, Campos MM
International immunopharmacology 2022 Jan;102:108386
International immunopharmacology 2022 Jan;102:108386
Agomelatine Softens Depressive-Like Behavior through the Regulation of Autophagy and Apoptosis.
Chen F, Chen S, Liu J, Amin N, Jin W, Fang M
BioMed research international 2021;2021:6664591
BioMed research international 2021;2021:6664591
Berberine Suppresses Mice Depression Behaviors and Promotes Hippocampal Neurons Growth Through Regulating the miR-34b-5p/miR-470-5p/BDNF Axis.
Zhan Y, Han J, Xia J, Wang X
Neuropsychiatric disease and treatment 2021;17:613-626
Neuropsychiatric disease and treatment 2021;17:613-626
Effect of C-phycocyanin on HDAC3 and miRNA-335 in Alzheimer's disease.
Li Z, Gan L, Yan S, Yan Y, Huang W
Translational neuroscience 2020;11(1):161-172
Translational neuroscience 2020;11(1):161-172
Intravenously Delivered Allogeneic Mesenchymal Stem Cells Bidirectionally Regulate Inflammation and Induce Neurotrophic Effects in Distal Middle Cerebral Artery Occlusion Rats Within the First 7 Days After Stroke.
Li X, Huang M, Zhao R, Zhao C, Liu Y, Zou H, Chen L, Guan Y, Zhang YA
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(5):1951-1970
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2018;46(5):1951-1970
Resveratrol prevents cognitive deficits induced by chronic unpredictable mild stress: Sirt1/miR-134 signalling pathway regulates CREB/BDNF expression in hippocampus in vivo and in vitro.
Shen J, Xu L, Qu C, Sun H, Zhang J
Behavioural brain research 2018 Sep 3;349:1-7
Behavioural brain research 2018 Sep 3;349:1-7
Influence of hippocampal low-frequency stimulation on GABA(A) R α1, ICER and BNDF expression level in brain tissues of amygdala-kindled drug-resistant temporal lobe epileptic rats.
Xu K, Liu Z, Wang L, Wu G, Liu T
Brain research 2018 Nov 1;1698:195-203
Brain research 2018 Nov 1;1698:195-203
Abnormal expressions of AGEs, TGF-β1, BDNF and their receptors in diabetic rat colon-Associations with colonic morphometric and biomechanical remodeling.
Sha H, Tong X, Zhao J
Scientific reports 2018 Jun 21;8(1):9437
Scientific reports 2018 Jun 21;8(1):9437
Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord.
Ma YH, Zeng X, Qiu XC, Wei QS, Che MT, Ding Y, Liu Z, Wu GH, Sun JH, Pang M, Rong LM, Liu B, Aljuboori Z, Han I, Ling EA, Zeng YS
Biomaterials 2018 Apr;160:37-55
Biomaterials 2018 Apr;160:37-55
The Proliferation Enhancing Effects of Salidroside on Schwann Cells In Vitro.
Liu H, Lv P, Wu H, Zhang K, Xu F, Zheng L, Zhao J
Evidence-based complementary and alternative medicine : eCAM 2017;2017:4673289
Evidence-based complementary and alternative medicine : eCAM 2017;2017:4673289
Effect of hippocampal L‑NBP on BDNF and TrkB expression and neurological function of vascular dementia rats.
Huang Y, Li Z, Nan G
Molecular medicine reports 2017 Nov;16(5):7673-7678
Molecular medicine reports 2017 Nov;16(5):7673-7678
Therapeutic efficacy of neural stem cells originating from umbilical cord-derived mesenchymal stem cells in diabetic retinopathy.
Zhang W, Wang Y, Kong J, Dong M, Duan H, Chen S
Scientific reports 2017 Mar 24;7(1):408
Scientific reports 2017 Mar 24;7(1):408
Synergistic effects of G-CSF and bone marrow stromal cells on nerve regeneration with acellular nerve xenografts.
Jia H, Wang Y, Wang T, Dong Y, Li WL, Li JP, Ma WZ, Tong XJ, He ZY
Synapse (New York, N.Y.) 2017 Jul;71(7)
Synapse (New York, N.Y.) 2017 Jul;71(7)
Electroacupuncture Ameliorates Learning and Memory and Improves Synaptic Plasticity via Activation of the PKA/CREB Signaling Pathway in Cerebral Hypoperfusion.
Zheng CX, Lu M, Guo YB, Zhang FX, Liu H, Guo F, Huang XL, Han XH
Evidence-based complementary and alternative medicine : eCAM 2016;2016:7893710
Evidence-based complementary and alternative medicine : eCAM 2016;2016:7893710
Ginkgo biloba and vitamin E ameliorate haloperidol-induced vacuous chewingmovement and brain-derived neurotrophic factor expression in a rat tardive dyskinesia model.
Shi J, Tan YL, Wang ZR, An HM, Li J, Wang YC, Lv MH, Yan SX, Wu JQ, Soares JC, Yang FD, Zhang XY
Pharmacology, biochemistry, and behavior 2016 Sep;148:53-8
Pharmacology, biochemistry, and behavior 2016 Sep;148:53-8
Dexmedetomidine protects against learning and memory impairments caused by electroconvulsive shock in depressed rats: Involvement of the NMDA receptor subunit 2B (NR2B)-ERK signaling pathway.
Gao X, Zhuang FZ, Qin SJ, Zhou L, Wang Y, Shen QF, Li M, Villarreal M, Benefield L, Gu SL, Ma TF
Psychiatry research 2016 Sep 30;243:446-52
Psychiatry research 2016 Sep 30;243:446-52
BDNF-induced expansion of cumulus-oocyte complexes in pigs was mediated by microRNA-205.
Li C, Chen C, Chen L, Chen S, Li H, Zhao Y, Rao J, Zhou X
Theriogenology 2016 May;85(8):1476-82
Theriogenology 2016 May;85(8):1476-82
Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene.
Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X
In vitro cellular & developmental biology. Animal 2015 Mar;51(3):319-27
In vitro cellular & developmental biology. Animal 2015 Mar;51(3):319-27
Protocatechuic acid improves cognitive deficits and attenuates amyloid deposits, inflammatory response in aged AβPP/PS1 double transgenic mice.
Song Y, Cui T, Xie N, Zhang X, Qian Z, Liu J
International immunopharmacology 2014 May;20(1):276-81
International immunopharmacology 2014 May;20(1):276-81
Changes in compressed neurons from dogs with acute and severe cauda equina constrictions following intrathecal injection of brain-derived neurotrophic factor-conjugated polymer nanoparticles.
Tan J, Shi J, Shi G, Liu Y, Liu X, Wang C, Chen D, Xing S, Shen L, Jia L, Ye X, He H, Li J
Neural regeneration research 2013 Jan 25;8(3):233-43
Neural regeneration research 2013 Jan 25;8(3):233-43
Sciatic nerve repair by acellular nerve xenografts implanted with BMSCs in rats xenograft combined with BMSCs.
Jia H, Wang Y, Tong XJ, Liu GB, Li Q, Zhang LX, Sun XH
Synapse (New York, N.Y.) 2012 Mar;66(3):256-69
Synapse (New York, N.Y.) 2012 Mar;66(3):256-69
Neural stem cells over-expressing brain-derived neurotrophic factor (BDNF) stimulate synaptic protein expression and promote functional recovery following transplantation in rat model of traumatic brain injury.
Ma H, Yu B, Kong L, Zhang Y, Shi Y
Neurochemical research 2012 Jan;37(1):69-83
Neurochemical research 2012 Jan;37(1):69-83
The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm.
Li C, Li C, Zhu X, Wang C, Liu Z, Li W, Lu C, Zhou X
Theriogenology 2012 Feb;77(3):636-43
Theriogenology 2012 Feb;77(3):636-43
Cellular and molecular mechanisms underlying the action of ginsenoside Rg1 against Alzheimer's disease.
Li X, Li M, Li Y, Quan Q, Wang J
Neural regeneration research 2012 Dec 25;7(36):2860-6
Neural regeneration research 2012 Dec 25;7(36):2860-6
Icariin isolated from Epimedium brevicornum Maxim attenuates learning and memory deficits induced by d-galactose in rats.
Li F, Gong QH, Wu Q, Lu YF, Shi JS
Pharmacology, biochemistry, and behavior 2010 Sep;96(3):301-5
Pharmacology, biochemistry, and behavior 2010 Sep;96(3):301-5
Acute stress and chronic stress change brain-derived neurotrophic factor (BDNF) and tyrosine kinase-coupled receptor (TrkB) expression in both young and aged rat hippocampus.
Shi SS, Shao SH, Yuan BP, Pan F, Li ZL
Yonsei medical journal 2010 Sep;51(5):661-71
Yonsei medical journal 2010 Sep;51(5):661-71
Protective effect of total flavones of Abelmoschus manihot L. Medic against poststroke depression injury in mice and its action mechanism.
Liu M, Jiang QH, Hao JL, Zhou LL
Anatomical record (Hoboken, N.J. : 2007) 2009 Mar;292(3):412-22
Anatomical record (Hoboken, N.J. : 2007) 2009 Mar;292(3):412-22
Enhanced neural differentiation of neural stem cells and neurite growth by amniotic epithelial cell co-culture.
Meng XT, Chen D, Dong ZY, Liu JM
Cell biology international 2007 Jul;31(7):691-8
Cell biology international 2007 Jul;31(7):691-8
Application of bFGF and BDNF to improve angiogenesis and cardiac function.
Liu Y, Sun L, Huan Y, Zhao H, Deng J
The Journal of surgical research 2006 Nov;136(1):85-91
The Journal of surgical research 2006 Nov;136(1):85-91
Catalpol increases hippocampal neuroplasticity and up-regulates PKC and BDNF in the aged rats.
Liu J, He QJ, Zou W, Wang HX, Bao YM, Liu YX, An LJ
Brain research 2006 Dec 6;1123(1):68-79
Brain research 2006 Dec 6;1123(1):68-79
Moclobemide up-regulates proliferation of hippocampal progenitor cells in chronically stressed mice.
Li YF, Zhang YZ, Liu YQ, Wang HL, Yuan L, Luo ZP
Acta pharmacologica Sinica 2004 Nov;25(11):1408-12
Acta pharmacologica Sinica 2004 Nov;25(11):1408-12
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of BDNF using anti-BDNF antibody (PB9075). BDNF was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BDNF Antibody (PB9075) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen. t
- Additional image
