H00000627-M02

antibody from Abnova Corporation

Targeting: BDNF

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000627-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000627-M02, RRID:AB_1136899

Product name

BDNF monoclonal antibody (M02), clone 1B10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant BDNF.

Antigen sequence

MTILFLTMVISYFGCMKAAPMKEANIRGQGGLAYP
GVRTHGTLESVNGPKAGSRGLTSLADTFEHMIEEL
LDEDQKVRPNEENNKDADLYTSRVMLSSQVPLEPP
LLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSV
CDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKG
QLKQYFYKTKCNPMGYAKEGCRGIDKRHWNSQCRT
TQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKR
GR


Isotype

IgG

Antibody clone number

1B10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BDNF expression in transfected 293T cell line by BDNF monoclonal antibody (M02), clone 1B10.Lane 1: BDNF transfected lysate(28.9 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to BDNF on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of BDNF transfected lysate using anti-BDNF monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BDNF monoclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol