Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-32783 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GCLM Recombinant Rabbit Monoclonal Antibody (JM39-61)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JM39-61
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Elevated type I interferon responses potentiate metabolic dysfunction, inflammation, and accelerated aging in mtDNA mutator mice.
Lei Y, Guerra Martinez C, Torres-Odio S, Bell SL, Birdwell CE, Bryant JD, Tong CW, Watson RO, West LC, West AP
Science advances 2021 May;7(22)
Science advances 2021 May;7(22)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GCLM in different lysates using a Monoclonal antibody (Product #MA5-32783) at a dilution of 1:500. Positive control: Lane 1: A431, Lane 2: PC-12, Lane 3: NIH-3T3.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GCLM in A431 cells using a GCLM Monoclonal antibody (Product # MA5-32783) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GCLM in A549 cells using a GCLM Monoclonal antibody (Product # MA5-32783) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of GCLM in Hela cells using a GCLM Monoclonal antibody (Product # MA5-32783) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GCLM of paraffin-embedded Human colon cancer tissue using a GCLM Monoclonal antibody (Product #MA5-32783). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GCLM of paraffin-embedded Human pancreas tissue using a GCLM Monoclonal antibody (Product #MA5-32783). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of GCLM of paraffin-embedded Mouse small intestine tissue using a GCLM Monoclonal antibody (Product #MA5-32783). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometric analysis of GCLM in Hela cells using a GCLM Monoclonal Antibody (Product # MA5-32783) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).