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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-22755 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-22755, RRID:AB_675582
- Product name
- Anti-GCLC
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Synthesis, biological evaluation, and structure-activity relationships of dithiolethiones as inducers of cytoprotective phase 2 enzymes.
5,6-Dihydrocyclopenta[c][1,2]-dithiole-3(4H)-thione is a promising cancer chemopreventive agent in the urinary bladder.
Cigarette smoking blocks the protective expression of Nrf2/ARE pathway in peripheral mononuclear cells of young heavy smokers favouring inflammation.
Clock and ATF4 transcription system regulates drug resistance in human cancer cell lines
Munday R, Zhang Y, Paonessa JD, Munday CM, Wilkins AL, Babu J
Journal of medicinal chemistry 2010 Jun 24;53(12):4761-7
Journal of medicinal chemistry 2010 Jun 24;53(12):4761-7
5,6-Dihydrocyclopenta[c][1,2]-dithiole-3(4H)-thione is a promising cancer chemopreventive agent in the urinary bladder.
Paonessa JD, Munday CM, Mhawech-Fauceglia P, Munday R, Zhang Y
Chemico-biological interactions 2009 Jun 15;180(1):119-26
Chemico-biological interactions 2009 Jun 15;180(1):119-26
Cigarette smoking blocks the protective expression of Nrf2/ARE pathway in peripheral mononuclear cells of young heavy smokers favouring inflammation.
Garbin U, Fratta Pasini A, Stranieri C, Cominacini M, Pasini A, Manfro S, Lugoboni F, Mozzini C, Guidi G, Faccini G, Cominacini L
PloS one 2009 Dec 9;4(12):e8225
PloS one 2009 Dec 9;4(12):e8225
Clock and ATF4 transcription system regulates drug resistance in human cancer cell lines
T Igarashi, H Izumi, T Uchiumi, K Nishio, T Arao, M Tanabe, H Uramoto, K Sugio, K Yasumoto, Y Sasaguri, K Y Wang, Y Otsuji, K Kohno
Oncogene 2007 Feb;26(33):4749-4760
Oncogene 2007 Feb;26(33):4749-4760
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 73
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
