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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP51800PU-N - Provider product page
- Provider
- OriGene
- Product name
- GCLC (N-term) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- GCLC (N-term) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- GCLC
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of GCLC / GLCLC?Antibody (N-term) ??in Jurkat cell line lysates (35ug/lane). This demonstrates the GCLC antibody detected the GCLC protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of GCLC / GLCLC?Antibody (N-term) ??with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma reacted with GCLC / GLCLC?Antibody (N-term) ??followed by peroxidase conjugation of the secondary antibody and DAB staining
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using GCLC / GLCLC?Antibody (N-term) ??(right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
