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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00002729-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00002729-M01, RRID:AB_2294387
- Product name
- Mouse Monoclonal GCLC Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. GCLC - glutamate-cysteine ligase, catalytic subunit
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Nuclear Factor Erythroid-Derived 2-Like 2-Induced Reductive Stress Favors Self-Renewal of Breast Cancer Stem-Like Cells via the FoxO3a-Bmi-1 Axis.
Polysulfide protects midbrain dopaminergic neurons from MPP+-induced degeneration via enhancement of glutathione biosynthesis.
Insulin-like growth factor 1 specifically up-regulates expression of modifier subunit of glutamate-cysteine ligase and enhances glutathione synthesis in SH-SY5Y cells.
Long-term Coexposure to Hexavalent Chromium and B[a]P Causes Tissue-Specific Differential Biological Effects in Liver and Gastrointestinal Tract of Mice.
Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.
Glycogen synthase kinase 3 regulates cell death and survival signaling in tumor cells under redox stress.
The LEGSKO mouse: a mouse model of age-related nuclear cataract based on genetic suppression of lens glutathione synthesis.
Bioactive food components prevent carcinogenic stress via Nrf2 activation in BRCA1 deficient breast epithelial cells.
Differential activation of the inflammasome in THP-1 cells exposed to chrysotile asbestos and Libby "six-mix" amphiboles and subsequent activation of BEAS-2B cells.
Protection against 2-chloroethyl ethyl sulfide (CEES)-induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway.
Diversity in antioxidant response enzymes in progressive stages of human nonalcoholic fatty liver disease.
Induction of avian musculoaponeurotic fibrosarcoma proteins by toxic bile acid inhibits expression of glutathione synthetic enzymes and contributes to cholestatic liver injury in mice.
Dysregulation of glutathione synthesis during cholestasis in mice: molecular mechanisms and therapeutic implications.
Kim DH, Jang JH, Kwon OS, Cha HJ, Youn HJ, Chun KS, Surh YJ
Antioxidants & redox signaling 2020 Jun;32(18):1313-1329
Antioxidants & redox signaling 2020 Jun;32(18):1313-1329
Polysulfide protects midbrain dopaminergic neurons from MPP+-induced degeneration via enhancement of glutathione biosynthesis.
Takahashi S, Hisatsune A, Kurauchi Y, Seki T, Katsuki H
Journal of pharmacological sciences 2018 May;137(1):47-54
Journal of pharmacological sciences 2018 May;137(1):47-54
Insulin-like growth factor 1 specifically up-regulates expression of modifier subunit of glutamate-cysteine ligase and enhances glutathione synthesis in SH-SY5Y cells.
Takahashi S, Hisatsune A, Kurauchi Y, Seki T, Katsuki H
European journal of pharmacology 2016 Jan 15;771:99-106
European journal of pharmacology 2016 Jan 15;771:99-106
Long-term Coexposure to Hexavalent Chromium and B[a]P Causes Tissue-Specific Differential Biological Effects in Liver and Gastrointestinal Tract of Mice.
Sánchez-Martín FJ, Fan Y, Carreira V, Ovesen JL, Vonhandorf A, Xia Y, Puga A
Toxicological sciences : an official journal of the Society of Toxicology 2015 Jul;146(1):52-64
Toxicological sciences : an official journal of the Society of Toxicology 2015 Jul;146(1):52-64
Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.
Sugiyama K, Ebinuma H, Nakamoto N, Sakasegawa N, Murakami Y, Chu PS, Usui S, Ishibashi Y, Wakayama Y, Taniki N, Murata H, Saito Y, Fukasawa M, Saito K, Yamagishi Y, Wakita T, Takaku H, Hibi T, Saito H, Kanai T
PloS one 2014;9(4):e94460
PloS one 2014;9(4):e94460
Glycogen synthase kinase 3 regulates cell death and survival signaling in tumor cells under redox stress.
Venè R, Cardinali B, Arena G, Ferrari N, Benelli R, Minghelli S, Poggi A, Noonan DM, Albini A, Tosetti F
Neoplasia (New York, N.Y.) 2014 Sep;16(9):710-22
Neoplasia (New York, N.Y.) 2014 Sep;16(9):710-22
The LEGSKO mouse: a mouse model of age-related nuclear cataract based on genetic suppression of lens glutathione synthesis.
Fan X, Liu X, Hao S, Wang B, Robinson ML, Monnier VM
PloS one 2012;7(11):e50832
PloS one 2012;7(11):e50832
Bioactive food components prevent carcinogenic stress via Nrf2 activation in BRCA1 deficient breast epithelial cells.
Kang HJ, Hong YB, Kim HJ, Wang A, Bae I
Toxicology letters 2012 Mar 7;209(2):154-60
Toxicology letters 2012 Mar 7;209(2):154-60
Differential activation of the inflammasome in THP-1 cells exposed to chrysotile asbestos and Libby "six-mix" amphiboles and subsequent activation of BEAS-2B cells.
Li M, Gunter ME, Fukagawa NK
Cytokine 2012 Dec;60(3):718-30
Cytokine 2012 Dec;60(3):718-30
Protection against 2-chloroethyl ethyl sulfide (CEES)-induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway.
Abel EL, Bubel JD, Simper MS, Powell L, McClellan SA, Andreeff M, MacLeod MC, DiGiovanni J
Toxicology and applied pharmacology 2011 Sep 1;255(2):176-83
Toxicology and applied pharmacology 2011 Sep 1;255(2):176-83
Diversity in antioxidant response enzymes in progressive stages of human nonalcoholic fatty liver disease.
Hardwick RN, Fisher CD, Canet MJ, Lake AD, Cherrington NJ
Drug metabolism and disposition: the biological fate of chemicals 2010 Dec;38(12):2293-301
Drug metabolism and disposition: the biological fate of chemicals 2010 Dec;38(12):2293-301
Induction of avian musculoaponeurotic fibrosarcoma proteins by toxic bile acid inhibits expression of glutathione synthetic enzymes and contributes to cholestatic liver injury in mice.
Yang H, Ko K, Xia M, Li TW, Oh P, Li J, Lu SC
Hepatology (Baltimore, Md.) 2010 Apr;51(4):1291-301
Hepatology (Baltimore, Md.) 2010 Apr;51(4):1291-301
Dysregulation of glutathione synthesis during cholestasis in mice: molecular mechanisms and therapeutic implications.
Yang H, Ramani K, Xia M, Ko KS, Li TW, Oh P, Li J, Lu SC
Hepatology (Baltimore, Md.) 2009 Jun;49(6):1982-91
Hepatology (Baltimore, Md.) 2009 Jun;49(6):1982-91
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GCLC Antibody (3H1) [H00002729-M01] - Analysis of GCLC expression in transfected 293T cell line by GCLC monoclonal antibody (M01), clone 3H1.Lane 1: GCLC transfected lysate(72.8 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GCLC Antibody (3H1) [H00002729-M01] - GCLC monoclonal antibody (M01), clone 3H1. Analysis of GCLC expression in PC-12.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GCLC Antibody (3H1) [H00002729-M01] - GCLC monoclonal antibody (M01), clone 3H1 Analysis of GCLC expression in A-431.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GCLC Antibody (3H1) [H00002729-M01] - Detection limit for recombinant GST tagged GCLC is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: GCLC Antibody (3H1) [H00002729-M01] - Analysis of monoclonal antibody to GCLC on HeLa cell. Antibody concentration 10 ug/ml.
