NBP1-87780

antibody from Novus Biologicals

Targeting: SERPINB5 maspin, PI5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-87780

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-87780, RRID:AB_11025397

Product name

Rabbit Polyclonal Serpin B5/Maspin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human Serpin B5/Maspin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis in human cell lines PC-3 and Caco-2 using anti-SERPINB5 antibody. Corresponding SERPINB5 RNA-seq data are presented for the same cell lines. Loading control: anti-HSP90B1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis using Anti-SERPINB5 antibody NBP1-87780 (A) shows similar pattern to independent antibody NBP1-87779 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis in HeLa cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human cerebral cortex, liver, skin and testis using Anti-SERPINB5 antibody NBP1-87780 (A) shows similar protein distribution across tissues to independent antibody NBP1-87779 (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human upper gastrointestinal shows weak to moderate cytoplasmic/membranous positivity in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human testis.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human liver.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human tonsil shows strong cytoplasmic positivity in squamous epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human skin shows strong positivity in squamous epithelial cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Analysis in human skin and skeletal muscle tissues. Corresponding SERPINB5 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Serpin B5/Maspin Antibody [NBP1-87780] - Staining of human cerebral cortex.