H00004436-D01P

antibody from Abnova Corporation

Targeting: MSH2 COCA1, HNPCC, HNPCC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004436-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004436-D01P, RRID:AB_1577454

Product name

MSH2 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human MSH2 protein.

Antigen sequence

MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVR
LFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPA
GAKNLQSVVLSKMNFESFVKDLLLVRQYRVEVYKN
RAGNKASKENDWYLAYKASPGNLSQFEDILFGNND
MSASIGVVGVKMSAVDGQRQVGVGYVDSIQRKLGL
CEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDM
GKLRQIIQRGGILITERKKADFSTKDIYQDLNRLL
KGKKGEQMNSAVLPEMENQVAVSSLSAVIKFLELL
SDDSNFGQFELTTFDFSQYMKLDIAAVRALNLFQG
SVEDTTGSQSLAALLNKCKTPQGQRLVNQWIKQPL
MDKNRIEERLNLVEAFVEDAELRQTLQEDLLRRFP
DLNRLAKKFQRQAANLQDCYRLYQGINQLPNVIQA
LEKHEGKHQKLLLAVFVTPLTDLRSDFSKFQEMIE
TTLDMDQVENHEFLVKPSFDPNLSELREIMNDLEK
KMQSTLISAARDLGLDPGKQIKLDSSAQFGYYFRV
TCKEEKVLRNNKNFSTVDIQKNGVKFTNSKLTSLN
EEYTKNKTEYEEAQDAIVKEIVNISSGYVEPMQTL
NDVLAQLDAVVSFAHVSNGAPVPYVRPAILEKGQG
RIILKASRHACVEVQDEIAFIPNDVYFEKDKQMFH
IITGPNMGGKSTYIRQTGVIVLMAQIGCFVPCESA
EVSIVDCILARVGAGDSQLKGVSTFMAEMLETASI
LRSATKDSLIIIDELGRGTSTYDGFGLAWAISEYI
ATKIGAFCMFATHFHELTALANQIPTVNNLHVTAL
TTEETLTMLYQVKKGVCDQSFGIHVAELANFPKHV
IECAKQKALELEEFQYIGESQGYDIMEPAAKKCYL
EREQGEKIIQEFLSKVKQMPFTEMSEENITIKLKQ
LKAEVIAKNNSFVNEIISRIKVTT


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MSH2 expression in transfected 293T cell line (H00004436-T01) by MSH2 MaxPab polyclonal antibody.Lane 1: MSH2 transfected lysate(104.70 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MSH2 MaxPab rabbit polyclonal antibody. Western Blot analysis of MSH2 expression in Hela S3 NE.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to MSH2 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MSH2 and MSH6. HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MSH6 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)