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Learn15520-1-AP
antibody from Invitrogen Antibodies
Targeting: MSH2
COCA1, HNPCC, HNPCC1
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [7]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 15520-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MSH2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SSGYVEPMQ TLNDVLAQLD AVVSFAHVSN GAPVPYVRPA ILEKGQGRII LKASRHACVE VQDEIAFIPN DVYFEKDKQM FHIITGPNMG GKSTYIRQTG VIVLMAQIGC FVPCESAEVS IVDCILARVG AGDSQLKGVS TFMAEMLETA SILRSATKDS LIIIDELGRG TSTYDGFGLA WAISEYIATK IGAFCMFATH FHELTALANQ IPTVNNLHVT ALTTEETLTM LYQVKKGVCD QSFGIHVAEL ANFPKHVIEC AKQKALELEE FQYIGESQGY DIMEPAAKKC YLEREQGEKI IQEFLSKVKQ MPFTEMSEEN ITIKLKQLKA EVIAKNNSFV NEIISRIKVT T (585-934 aa encoded by BC021566)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB results of MSH2 (15520-1-AP, 1:5000) antibody with si-Control and si-MSH2 transfected HeLa cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A2780 cells were subjected to SDS PAGE followed by western blot with 15520-1-AP (MSH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using MSH2 antibody 15520-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human tonsil using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human colon cancer using 15520-1-AP (MSH2 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug MSH2 antibody (15520-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-MSH2 (IP:15520-1-AP, 5ug; Detection:15520-1-AP 1:500) with HeLa cells lysate 2000ug.
