Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2877 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2877, RRID:AB_1574128
- Product name
- CSNK1E polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CSNK1E.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Casein kinase I epsilon enhances the binding of Dvl-1 to Frat-1 and is essential for Wnt-3a-induced accumulation of beta-catenin.
The circadian regulatory proteins BMAL1 and cryptochromes are substrates of casein kinase Iepsilon.
Phosphorylation and destabilization of human period I clock protein by human casein kinase I epsilon.
Hino S, Michiue T, Asashima M, Kikuchi A
The Journal of biological chemistry 2003 Apr 18;278(16):14066-73
The Journal of biological chemistry 2003 Apr 18;278(16):14066-73
The circadian regulatory proteins BMAL1 and cryptochromes are substrates of casein kinase Iepsilon.
Eide EJ, Vielhaber EL, Hinz WA, Virshup DM
The Journal of biological chemistry 2002 May 10;277(19):17248-54
The Journal of biological chemistry 2002 May 10;277(19):17248-54
Phosphorylation and destabilization of human period I clock protein by human casein kinase I epsilon.
Keesler GA, Camacho F, Guo Y, Virshup D, Mondadori C, Yao Z
Neuroreport 2000 Apr 7;11(5):951-5
Neuroreport 2000 Apr 7;11(5):951-5
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CSNK1E polyclonal antibody (Cat # PAB2877) in SK-BR-3 cell lysate. CSNK1E (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CSNK1E polyclonal antibody (Cat # PAB2877) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)