HPA031677

antibody from Atlas Antibodies

Targeting: MYLK MLCK, MLCK1, MYLK1, smMLCK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA031677

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA031677, RRID:AB_10794617

Product name

Anti-MYLK

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKS
RRPKSSLPPVLGTESDATVKKKPAPKTPPKAAMPP
QIIQFPEDQKVRAGESVELFGKVTGTQPITCTWMK
FRKQIQESEHMKVENSENGSKLTILAARQEHCGCY
TLLVE


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line HEL

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human smooth muscle and skeletal muscle tissues using Anti-MYLK antibody. Corresponding MYLK RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human smooth muscle shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN