PAB3181

antibody from Abnova Corporation

Targeting: MYLK MLCK, MLCK1, MYLK1, smMLCK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3181

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3181, RRID:AB_1577297

Product name

MYLK polyclonal antibody

Antibody type

Polyclonal

Antigen

A synthetic peptide (conjugated with KLH) corresponding to amino acids 40-69 at N-terminus of human MYLK.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

400 μl

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Oncogenic kinase signalling.

Blume-Jensen P, Hunter T
Nature 2001 May 17;411(6835):355-65

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Phosphoinositide 3-kinase signalling pathways.

Cantrell DA
Journal of cell science 2001 Apr;114(Pt 8):1439-45

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The RET proto-oncogene in human cancers.

Jhiang SM
Oncogene 2000 Nov 20;19(49):5590-7

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of MYLK polyclonal antibody (Cat # PAB3181) in A-375 cell lysate. MYLK (Arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with MYLK polyclonal antibody (Cat # PAB3181) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)