A01697-2

antibody from Boster Biological Technology

Targeting: MYLK MLCK, MLCK1, MYLK1, smMLCK

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A01697-2

Provider

Boster Biological Technology

Product name

Anti-Myosin light chain kinase/MYLK Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for MYLK detection. Tested with WB, IHC-P, Direct ELISA in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Extreme Diversity of the Human Vascular Mesenchymal Cell Landscape.

Bruijn LE, van den Akker BEWM, van Rhijn CM, Hamming JF, Lindeman JHN
Journal of the American Heart Association 2020 Dec;9(23):e017094

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of MYLK using anti-MYLK antibody (A01697-2). MYLK was detected in paraffin-embedded section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-MYLK Antibody (A01697-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image