MA5-32136

antibody from Invitrogen Antibodies

Targeting: MYLK MLCK, MLCK1, MYLK1, smMLCK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32136

Provider

Invitrogen Antibodies

Product name

MYLK Recombinant Rabbit Monoclonal Antibody (SU40-06)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

SU40-06

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MYLK in human lung cell lysate using a MYLK Monoclonal antibody (Product # MA5-32136) at a dilution of 1:5,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of MYLK in Hela cells using a MYLK Monoclonal antibody (Product # MA5-32136) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of MYLK in L6 cells using a MYLK Monoclonal antibody (Product # MA5-32136) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of MYLK in NIH/3T3 cells using a MYLK Monoclonal antibody (Product # MA5-32136) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of MYLK in SH-SY-5Y cells using a MYLK Monoclonal antibody (Product # MA5-32136) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MYLK of paraffin-embedded rat smooth muscle tissue using a MYLK Monoclonal antibody (Product #MA5-32136). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MYLK of paraffin-embedded Human colon cancer tissue using a MYLK Monoclonal antibody (Product #MA5-32136). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MYLK of paraffin-embedded Mouse smooth muscle tissue using a MYLK Monoclonal antibody (Product #MA5-32136). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of MYLK in SH-SY-5Y cells using a MYLK Monoclonal Antibody (Product # MA5-32136) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.