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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX23561 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX23561, RRID:AB_374359
- Product name
- Cannabinoid receptor 2 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Opposite effects of cannabinoid CB(1) and CB(2) receptors on antipsychotic clozapine-induced cardiotoxicity.
Li L, Dong X, Tu C, Li X, Peng Z, Zhou Y, Zhang D, Jiang J, Burke A, Zhao Z, Jin L, Jiang Y
British journal of pharmacology 2019 Apr;176(7):890-905
British journal of pharmacology 2019 Apr;176(7):890-905
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of Cannabinoid Receptor II in 25 ug of HT29, C6 and rat colon lysates. Proteins were transferred to a PVDF membrane and blocked at 4¢XC overnight. The membrane was probed with Cannabinoid Receptor II antibody at a dilution of 1:200 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cannabinoid Receptor II in AtT20 cells transfected with the rat CB2 gene.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Cannabinoid Receptor II in paraffin-embedded human skin tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Cannabinoid Receptor II antibody diluted by 3% BSA-PBS at a dilution of 1:20 overnight at 4¢XC in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
