AF5587

antibody from R&D Systems

Targeting: STEAP1 PRSS24, STEAP

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5587

Provider

R&D Systems

Product name

Human STEAP1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human STEAP1 in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9UHE8


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human STEAP1 by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human STEAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5587) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for STEAP1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human STEAP1 by Simple Western^TM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for STEAP1 at approximately 40 kDa (as indicated) using 10 µg/mL of Goat Anti-Human STEAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5587) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.