MA5-32229

antibody from Invitrogen Antibodies

Targeting: DKK1 DKK-1, SK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32229

Provider

Invitrogen Antibodies

Product name

DKK1 Recombinant Rabbit Monoclonal Antibody (SC06-86)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Reactivity

Human, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

SC06-86

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The Influence of Pro-Inflammatory Factors on Sclerostin and Dickkopf-1 Production in Human Dental Pulp Cells Under Hypoxic Conditions.

Janjić K, Samiei M, Moritz A, Agis H
Frontiers in bioengineering and biotechnology 2019;7:430

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DKK1 in MCF-7 cell lysate using a DKK1 Monoclonal antibody (Product # MA5-32229) at a dilution of 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-DKK1 Recombinant Rabbit Monoclonal Antibody (SC06-86) (Product # MA5-32229) and a 28-40 kDa band corresponding to DKK1 was observed in HCT 116 treated with Protein transport inhibitor (1X for 4hrs) but not in U-937 cells which is reported to low or negative for DKK1 expression. Additional uncharacterized bands (*) were observed across the cell lines. Whole-cell extracts (30 µg lysate) of HCT 116 (Lane 1), HCT 116 treated with Protein transport inhibitor (1X for 4hrs) (Lane 2), U-937 (Lane 3), U-937 treated with Protein transport inhibitor (1X for 4hrs) (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of DKK1 in Hela cells using a DKK1 Monoclonal antibody (Product # MA5-32229) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of DKK1 in HepG2 cells using a DKK1 Monoclonal antibody (Product # MA5-32229) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of DKK1 in NCCIT cells using a DKK1 Monoclonal antibody (Product # MA5-32229) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of DKK1 of paraffin-embedded Human uterus tissue using a DKK1 Monoclonal antibody (Product #MA5-32229). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of DKK1 in Hela cells using a DKK1 Monoclonal Antibody (Product # MA5-32229) at a dilution of 1:50, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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