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LearnPA5-18233
antibody from Invitrogen Antibodies
Targeting: PARD6A
PAR-6, PAR-6A, PAR6alpha, TAX40, TIP-40
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- PA5-18233 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAR6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.
Peng XM, Gao S, Deng HT, Cai HX, Zhou Z, Xiang R, Zhang QZ, Li LY
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Oct;32(10):5577-5586
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 Oct;32(10):5577-5586
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PAR6 by a PAR6 monoclonal antibody (Product # PA5-18233) at a concentration of 2 µg/mL. Jurkat (A) and U251 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded of Human Heart using Product # PA5-18233 at a concentration of 5 µg/mL. The tissue was processed by steamed antigen retrieval with citrate buffer pH 6 and stained with AP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded of Human Pancreas using Product # PA5-18233 at a concentration of 10 µg/mL. The tissue was processed by microwaved antigen retrieval with Tris/EDTA buffer pH 9 and stained with HRP.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PAR6 in Jurkat cells using a PAR6 monoclonal antibody (Product # PA5-18233) at 10 µg/mL for 1hr, depicted by the blue line. The cells were paraformaldehyde fixed and permeabilized with 0.5% Triton. Primary incubation followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of PAR6 in Jurkat cells using a polyclonal antibody (Product #PA5-18233). Jurkat cells (blue line) were paraformaldehyde fixed and permeabilized with 0.5% Triton. The primary antibody was incubated for one hour (10 µg/mL) followed by an Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by an Alexa Fluor 488 secondary antibody.
