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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MA5-47281 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Synapsin II Monoclonal Antibody (GT1446)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Store as concentrated solution.
- Antibody clone number
- GT1446
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Synapsin II in Various tissue extracts (50 µg) separated by 7.5% SDS-PAGE. Membrane was blotted with Synapsin II Monoclonal antibody (Product # MA5-47281) using a dilution of 1:10,000 followed by HRP-conjugated anti-mouse IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Synapsin II in DIV10 rat E18 primary cortical neuron cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Synapsin II stained by Synapsin II monoclonal antibody (Product # MA5-47281) diluted at 1:500. Red: Tau, a axon marker, stained by Tau antibody diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Synapsin II in paraffin-embedded mouse eye. Green: Synapsin II stained by Synapsin II monoclonal antibody (Product # MA5-47281) diluted at 1:250. Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
