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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB12022 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12022, RRID:AB_1714616
- Product name
- SPTBN2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant SPTBN2.
- Storage
- Store at 4°C. Do not freeze.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of SPTBN2 in HeLa whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK 293T (Bosc 23) (T : 50 ug) cells with SPTBN2 polyclonal antibody (Cat # PAB12022).Antibodies : SPTBN2 polyclonal antibody (Cat # PAB12022) used for WB at 0.04 ug/mL (A) and 1 ug/mL (B) and used for IP at 3 ug/mg lysate.SPTBN2 was also immunoprecipitated by SPTBN2 polyclonal antibody (Cat # PAB12023), which recognizes an upstream epitope.Detection : Chemiluminescence with exposure times of 10 seconds (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of SPTBN2 in human prostate epithelium with SPTBN2 polyclonal antibody (Cat # PAB12022).
- Validation comment
- Immunohistochemistry
