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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6192 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6192, RRID:AB_1576642
- Product name
- KIF5B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of KIF5B.
- Antigen sequence
C-QPVAVRGGGGKQV- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Conventional kinesin KIF5B mediates insulin-stimulated GLUT4 movements on microtubules.
Semiz S, Park JG, Nicoloro SM, Furcinitti P, Zhang C, Chawla A, Leszyk J, Czech MP
The EMBO journal 2003 May 15;22(10):2387-99
The EMBO journal 2003 May 15;22(10):2387-99
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KIF5B polyclonal antibody (Cat # PAB6192) staining (0.2 ug/mL) of human kidney lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KIF5B polyclonal antibody (Cat # PAB6192) staining (1 ug/mL) of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
