sc-28612

antibody from Santa Cruz Biotechnology

Targeting: RORA NR1F1, ROR1, ROR2, ROR3, RZRA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-28612

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-28612, RRID:AB_2180141

Product name

Anti-RORA

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

References

Submitted references

Partial loss of Tip60 slows mid-stage neurodegeneration in a spinocerebellar ataxia type 1 (SCA1) mouse model.

Gehrking KM, Andresen JM, Duvick L, Lough J, Zoghbi HY, Orr HT
Human molecular genetics 2011 Jun 1;20(11):2204-12

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Sex hormones in autism: androgens and estrogens differentially and reciprocally regulate RORA, a novel candidate gene for autism.

Sarachana T, Xu M, Wu RC, Hu VW
PloS one 2011 Feb 16;6(2):e17116

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.

Primary Ab dilution

1:500

Secondary Ab dilution

1:3000

Lane 1

Marker [kDa]: 219, 111, 83, 48, 32, 26, 17

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 62

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells and decidual cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.