Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [4]
- Flow cytometry [1]
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Validation data
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- Product number
- BS-4611R - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AQP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney lysates probed with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:300 in 4°C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90 min in 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat kidney lysates probed with Aquaporine 2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Kidney lysates probed with AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37°C.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Image provided by Independent Validation program, badge number 029457:Formalin-fixed and paraffin embedded human breast labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-AQP2 Polyclonal Antibody, unconjugated (bs-4611R) at 1:200 overnight at 37°C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) at 1:200, 40 minutes at 37°C. DAPI(5 µg/mL,blue,C-0033) was used to stain the cell nuclei.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Image provided the Independent Validation Program, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Image provided by Independent Validation program, badge number 029457:Formalin-fixed and paraffin embedded human breast labeled with Anti-AQP2 Polyclonal Antibody, Unconjugated (bs-4611R) at 1:250 followed by conjugation to the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney cells probed with Rabbit Anti-AQP2 Polyclonal Antibody (bs-4611R) at 1:50 for 40 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) FITC Conjugated Secondary Antibody.