Antibody data
- Antibody Data
- Antigen structure
- References [22]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- NB110-74682 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-74682, RRID:AB_1107363
- Product name
- Rabbit Polyclonal Aquaporin-2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 0.16 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references "Reversed polarization" of Na/K-ATPase-a sign of inverted transport in the human endolymphatic sac: a super-resolution structured illumination microscopy (SR-SIM) study.
Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells.
The profibrotic effects of MK-8617 on tubulointerstitial fibrosis mediated by the KLF5 regulating pathway.
Zhen-wu-tang attenuates Adriamycin-induced nephropathy via regulating AQP2 and miR-92b.
Urinary Angiotensinogen Predicts Renal Disease Activity in Lupus Nephritis.
Different Effects of Tolvaptan in Patients with Idiopathic Membranous Nephropathy with Nephrotic Syndrome.
Parathyroid hormone controls paracellular Ca2+ transport in the thick ascending limb by regulating the tight-junction protein Claudin14.
Piezo1-dependent regulation of urinary osmolarity.
Expression of a dominant negative PKA mutation in the kidney elicits a diabetes insipidus phenotype.
Deep proteomic profiling of vasopressin-sensitive collecting duct cells. II. Bioinformatic analysis of vasopressin signaling.
Effect of tolvaptan in patients with chronic kidney disease due to diabetic nephropathy with heart failure.
Effect of tolvaptan in patients with chronic kidney disease due to diabetic nephropathy with heart failure.
RiboTag analysis of actively translated mRNAs in Sertoli and Leydig cells in vivo.
A novel model of surgical injury in adult rat kidney: a "pouch model".
Rapamycin inhibition of mTORC1 reverses lithium-induced proliferation of renal collecting duct cells.
Localization of aquaporins in the mouse vestibular end organs.
Deficiency of the calcium-sensing receptor in the kidney causes parathyroid hormone-independent hypocalciuria.
Localization of aquaporins 1, 2, and 3 and vasopressin type 2 receptor in the mouse inner ear.
AT1 receptors in the collecting duct directly modulate the concentration of urine.
Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells.
Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct.
Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2.
Nordström CK, Danckwardt-Lillieström N, Liu W, Rask-Andersen H
Cell and tissue research 2020 Mar;379(3):445-457
Cell and tissue research 2020 Mar;379(3):445-457
Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells.
Hariharan K, Reinke P, Kurtz A
Methods in molecular biology (Clifton, N.J.) 2019;1926:103-115
Methods in molecular biology (Clifton, N.J.) 2019;1926:103-115
The profibrotic effects of MK-8617 on tubulointerstitial fibrosis mediated by the KLF5 regulating pathway.
Li ZL, Lv LL, Wang B, Tang TT, Feng Y, Cao JY, Jiang LQ, Sun YB, Liu H, Zhang XL, Ma KL, Tang RN, Liu BC
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Nov;33(11):12630-12643
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 Nov;33(11):12630-12643
Zhen-wu-tang attenuates Adriamycin-induced nephropathy via regulating AQP2 and miR-92b.
Liang CL, Zhang PC, Wu JB, Liu BH, Yu-He, Lu RR, Jie-Zhou, Zhou JY
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Jan;109:1296-1305
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Jan;109:1296-1305
Urinary Angiotensinogen Predicts Renal Disease Activity in Lupus Nephritis.
Shi M, Luo W, Feng X, Jin L, Yang M, Wu L, Yang Z, Su C, Li Y, Su H, Wang G, Cao W
Antioxidants & redox signaling 2019 Dec;31(17):1289-1301
Antioxidants & redox signaling 2019 Dec;31(17):1289-1301
Different Effects of Tolvaptan in Patients with Idiopathic Membranous Nephropathy with Nephrotic Syndrome.
Tanaka A, Nakamura T, Sato E, Ueda Y, Node K
Internal medicine (Tokyo, Japan) 2017;56(2):191-196
Internal medicine (Tokyo, Japan) 2017;56(2):191-196
Parathyroid hormone controls paracellular Ca2+ transport in the thick ascending limb by regulating the tight-junction protein Claudin14.
Sato T, Courbebaisse M, Ide N, Fan Y, Hanai JI, Kaludjerovic J, Densmore MJ, Yuan Q, Toka HR, Pollak MR, Hou J, Lanske B
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 18;114(16):E3344-E3353
Proceedings of the National Academy of Sciences of the United States of America 2017 Apr 18;114(16):E3344-E3353
Piezo1-dependent regulation of urinary osmolarity.
Martins JR, Penton D, Peyronnet R, Arhatte M, Moro C, Picard N, Kurt B, Patel A, Honoré E, Demolombe S
Pflugers Archiv : European journal of physiology 2016 Jul;468(7):1197-1206
Pflugers Archiv : European journal of physiology 2016 Jul;468(7):1197-1206
Expression of a dominant negative PKA mutation in the kidney elicits a diabetes insipidus phenotype.
Gilbert ML, Yang L, Su T, McKnight GS
American journal of physiology. Renal physiology 2015 Mar 15;308(6):F627-38
American journal of physiology. Renal physiology 2015 Mar 15;308(6):F627-38
Deep proteomic profiling of vasopressin-sensitive collecting duct cells. II. Bioinformatic analysis of vasopressin signaling.
Yang CR, Raghuram V, Emamian M, Sandoval PC, Knepper MA
American journal of physiology. Cell physiology 2015 Dec 15;309(12):C799-812
American journal of physiology. Cell physiology 2015 Dec 15;309(12):C799-812
Effect of tolvaptan in patients with chronic kidney disease due to diabetic nephropathy with heart failure.
Sato E, Nakamura T, Amaha M, Nomura M, Matsumura D, Yamagishi H, Ono Y, Ueda Y
International heart journal 2014;55(6):533-8
International heart journal 2014;55(6):533-8
Effect of tolvaptan in patients with chronic kidney disease due to diabetic nephropathy with heart failure.
Sato E, Nakamura T, Amaha M, Nomura M, Matsumura D, Yamagishi H, Ono Y, Ueda Y
International heart journal 2014;55(6):533-8
International heart journal 2014;55(6):533-8
RiboTag analysis of actively translated mRNAs in Sertoli and Leydig cells in vivo.
Sanz E, Evanoff R, Quintana A, Evans E, Miller JA, Ko C, Amieux PS, Griswold MD, McKnight GS
PloS one 2013;8(6):e66179
PloS one 2013;8(6):e66179
A novel model of surgical injury in adult rat kidney: a "pouch model".
Litbarg NO, Vujicic S, Setty S, Sethupathi P, Dunea G, Arruda JA, Singh AK
Scientific reports 2013 Oct 8;3:2890
Scientific reports 2013 Oct 8;3:2890
Rapamycin inhibition of mTORC1 reverses lithium-induced proliferation of renal collecting duct cells.
Gao Y, Romero-Aleshire MJ, Cai Q, Price TJ, Brooks HL
American journal of physiology. Renal physiology 2013 Oct 15;305(8):F1201-8
American journal of physiology. Renal physiology 2013 Oct 15;305(8):F1201-8
Localization of aquaporins in the mouse vestibular end organs.
Takumida M, Takumida H, Kakigi A, Egami N, Nishioka R, Anniko M
Acta oto-laryngologica 2013 Aug;133(8):804-13
Acta oto-laryngologica 2013 Aug;133(8):804-13
Deficiency of the calcium-sensing receptor in the kidney causes parathyroid hormone-independent hypocalciuria.
Toka HR, Al-Romaih K, Koshy JM, DiBartolo S 3rd, Kos CH, Quinn SJ, Curhan GC, Mount DB, Brown EM, Pollak MR
Journal of the American Society of Nephrology : JASN 2012 Nov;23(11):1879-90
Journal of the American Society of Nephrology : JASN 2012 Nov;23(11):1879-90
Localization of aquaporins 1, 2, and 3 and vasopressin type 2 receptor in the mouse inner ear.
Takumida M, Kakigi A, Egami N, Nishioka R, Anniko M
Acta oto-laryngologica 2012 Aug;132(8):807-13
Acta oto-laryngologica 2012 Aug;132(8):807-13
AT1 receptors in the collecting duct directly modulate the concentration of urine.
Stegbauer J, Gurley SB, Sparks MA, Woznowski M, Kohan DE, Yan M, Lehrich RW, Coffman TM
Journal of the American Society of Nephrology : JASN 2011 Dec;22(12):2237-46
Journal of the American Society of Nephrology : JASN 2011 Dec;22(12):2237-46
Serine 269 phosphorylated aquaporin-2 is targeted to the apical membrane of collecting duct principal cells.
Moeller HB, Knepper MA, Fenton RA
Kidney international 2009 Feb;75(3):295-303
Kidney international 2009 Feb;75(3):295-303
Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct.
Yu MJ, Miller RL, Uawithya P, Rinschen MM, Khositseth S, Braucht DW, Chou CL, Pisitkun T, Nelson RD, Knepper MA
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 17;106(7):2441-6
Proceedings of the National Academy of Sciences of the United States of America 2009 Feb 17;106(7):2441-6
Vasopressin-stimulated increase in phosphorylation at Ser269 potentiates plasma membrane retention of aquaporin-2.
Hoffert JD, Fenton RA, Moeller HB, Simons B, Tchapyjnikov D, McDill BW, Yu MJ, Pisitkun T, Chen F, Knepper MA
The Journal of biological chemistry 2008 Sep 5;283(36):24617-27
The Journal of biological chemistry 2008 Sep 5;283(36):24617-27
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aquaporin-2 Antibody [NB110-74682] - Detection of Aquaporin 2 in rat kidney extracts. Cortex (CX), Outer Medulla (OM), Inner Medulla Base (IMB), Inner Medulla Papilla (IMP).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Aquaporin-2 Antibody [NB110-74682] - Analysis of FFPE human kidney using 1:100 conc. of Aquaporin-2 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Lumenal staining of Aquaporin-2 in scattered tubules was observed. Staining was performed by Histowiz.