Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- GTX114209 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114209, RRID:AB_11179808
- Product name
- SFPQ antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE GTX114209 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: JC B: BCL-1 7.5% SDS PAGE GTX114209 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SFPQ antibody detects SFPQ protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody (GTX114209) diluted by 1:10000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SFPQ antibody detects SFPQ protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody (GTX114209) diluted by 1:10000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SFPQ antibody detects SFPQ protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SFPQ antibody (GTX114209) diluted by 1:10000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SFPQ antibody [N2C1], Internal detects SFPQ protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SFPQ protein stained by SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of SFPQ protein from 293T whole cell extracts using 5 £gg of SFPQ antibody [N2C1], Internal (GTX114209).Western blot analysis was performed using SFPQ antibody [N2C1], Internal (GTX114209).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Hela xenograft, using SFPQ(GTX114209) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded mouse uterus, using SFPQ(GTX114209) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SFPQ antibody [N2C1], Internal detects SFPQ protein at cytoplasm and nucleus in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. SFPQ antibody [N2C1], Internal (GTX114209) diluted at 1:500.