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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00006421-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00006421-M02, RRID:AB_2186926
- Product name
- Mouse Monoclonal SFPQ Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. SFPQ - splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Novel snail1 target proteins in human colon cancer identified by proteomic analysis.
A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4.
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Larriba MJ, Casado-Vela J, Pendás-Franco N, Peña R, García de Herreros A, Berciano MT, Lafarga M, Casal JI, Muñoz A
PloS one 2010 Apr 20;5(4):e10221
PloS one 2010 Apr 20;5(4):e10221
A cell-based screen for splicing regulators identifies hnRNP LL as a distinct signal-induced repressor of CD45 variable exon 4.
Topp JD, Jackson J, Melton AA, Lynch KW
RNA (New York, N.Y.) 2008 Oct;14(10):2038-49
RNA (New York, N.Y.) 2008 Oct;14(10):2038-49
Combinatorial control of signal-induced exon repression by hnRNP L and PSF.
Melton AA, Jackson J, Wang J, Lynch KW
Molecular and cellular biology 2007 Oct;27(19):6972-84
Molecular and cellular biology 2007 Oct;27(19):6972-84
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SFPQ Antibody (6D7) [H00006421-M02] - SFPQ monoclonal antibody (M02), clone 6D7 Analysis of SFPQ expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SFPQ Antibody (6D7) [H00006421-M02] - Detection limit for recombinant GST tagged SFPQ is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: SFPQ Antibody (6D7) [H00006421-M02] - Analysis of monoclonal antibody to SFPQ on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SFPQ Antibody (6D7) [H00006421-M02] - Analysis of monoclonal antibody to SFPQ on formalin-fixed paraffin-embedded human thyroid nodular goiter. Antibody concentration 3 ug/ml.
